Das P K, Sayer K
Enzyme. 1975;19(4):225-32. doi: 10.1159/000458995.
Homogenates of rat jejunum and heart tissue in 0.3 M sucrose have been separated into different subcellular fractions by centrifugation. The distribution of cholinesterases in these two tissues is different. Acetylthiocholine (Ac), propionylthiocholine (Pc), and butyrylthiocholine (Bc) iodides were used as substrates. Using 1 mmol/1 Ac in the presence of hexafluorenium, a local anaesthetic drug, inhibition percentages of these subcellular cholinesterases were found to be different. The differences in the effect of hexafluorenium were most marked at a concentration of 5 mumol/1. Four electrophoretically distinct subcomponents of cholinesterase have been identified in different subcellular fractions from both jejunum and heart tissues. The electrophoretic profile, as determined by polyacrylamide disc electrophoresis, of different subcellular cholinesterases in these two tissues also appears to be different.
将大鼠空肠和心脏组织在0.3M蔗糖中制成的匀浆通过离心分离成不同的亚细胞组分。这两种组织中胆碱酯酶的分布不同。使用碘化乙酰硫代胆碱（Ac）、碘化丙酰硫代胆碱（Pc）和碘化丁酰硫代胆碱（Bc）作为底物。在局部麻醉药六氟铵存在的情况下，使用1mmol/1的Ac，发现这些亚细胞胆碱酯酶的抑制百分比不同。六氟铵在5μmol/1浓度时的作用差异最为明显。在空肠和心脏组织的不同亚细胞组分中已鉴定出四种电泳性质不同的胆碱酯酶亚组分。通过聚丙烯酰胺圆盘电泳测定，这两种组织中不同亚细胞胆碱酯酶的电泳图谱似乎也不同。
