[特发性血小板减少性紫癜患者中血小板生成素相关基因的表达]
[Expression of thrombopoietin related genes in patients with idiopathic thrombocytopenic purpura].
作者信息
Zhang X, Yang Y, Shi H
机构信息
Institute of Hematology, Beijing Medical University, Beijing 100044.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 1998 Nov;19(11):587-9.
OBJECTIVE
To explore the expression of thrombopoietin (Tpo) and its receptor c-mpl in bone marrow from patients with idiopathic thrombocytopenic purpura(ITP).
METHODS
Non-radioactive in situ hybridization was used to detect tpo, c-mpl mRNA. Immune cytochemistry was used to detect MPL on megakaryocyte.
RESULTS
The expression of tpo, c-mpl mRNA in bone marrow and MPL on megakaryocyte in patients with ITP was increased.
CONCLUSION
c-mpl mRNA was regulated at transcriptional level. A negative feedback loop was involved in the regulation of megakaryopoiesis in bone marrow. MPL may be involved in the regulation of circulating Tpo.
目的
探讨血小板生成素（Tpo）及其受体c-mpl在特发性血小板减少性紫癜（ITP）患者骨髓中的表达情况。
方法
采用非放射性原位杂交法检测tpo、c-mpl mRNA。采用免疫细胞化学法检测巨核细胞上的MPL。
结果
ITP患者骨髓中tpo、c-mpl mRNA的表达及巨核细胞上MPL的表达均增加。
结论
c-mpl mRNA在转录水平受到调控。骨髓巨核细胞生成的调控涉及负反馈环。MPL可能参与循环Tpo的调控。
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