Bock Oliver, Schlué Jerome, Mengel Michael, Büsche Guntram, Serinsöz Ebru, Kreipe Hans
Institute of Pathology, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
J Pathol. 2004 May;203(1):609-15. doi: 10.1002/path.1558.
The thrombopoietin receptor (Mpl) is involved in the pathogenesis of chronic myeloproliferative disorders (CMPD). In this study, we determined Mpl expression by bone marrow cells and megakaryocytes in CMPD by applying laser microdissection, real-time RT-PCR, and immunohistochemistry. Mpl mRNA expression was significantly increased up to 9-fold in total bone marrow cells (p < 0.001) and up to 4-fold in megakaryocytes in chronic myeloproliferative disorders (n = 73) compared to normal controls (n = 26, p = 0.01). Immunohistochemistry revealed heterogeneous Mpl expression by megakaryocytes in CMPD with a stronger accentuation in idiopathic myelofibrosis (IMF) in comparison to polycythaemia vera (PV) and essential thrombocythemia (ET). In addition to megakaryocytes, the erythropoietic lineage was prominently labelled by Mpl antiserum, with considerably stronger staining in polycythaemia vera. We conclude that, in CMPD, megakaryocytes and erythroid cells exhibit increased Mpl expression levels which may contribute to the sustained proliferation of both cell lineages in CMPD.
血小板生成素受体（Mpl）参与慢性骨髓增殖性疾病（CMPD）的发病机制。在本研究中，我们通过激光显微切割、实时逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法，测定了CMPD患者骨髓细胞和巨核细胞中的Mpl表达。与正常对照（n = 26，p = 0.01）相比，慢性骨髓增殖性疾病（n = 73）患者的全骨髓细胞中Mpl mRNA表达显著增加高达9倍（p < 0.001），巨核细胞中增加高达4倍。免疫组织化学显示，CMPD患者巨核细胞的Mpl表达异质性，与真性红细胞增多症（PV）和原发性血小板增多症（ET）相比，特发性骨髓纤维化（IMF）中Mpl表达更强。除巨核细胞外，红系细胞也被Mpl抗血清显著标记，在真性红细胞增多症中染色明显更强。我们得出结论，在CMPD中，巨核细胞和红系细胞的Mpl表达水平升高，这可能有助于CMPD中两个细胞系的持续增殖。
