Tang S, Bao X, Zheng T
Department of Heredity, Second Hospital of Wenzhen, Wenzhen 325000.
Zhonghua Jie He He Hu Xi Za Zhi. 1998 Mar;21(3):178-80.
To evaluate the clinical value of detection of Mycobacterium tuberculosis DNA (MTB-DNA) in peripheral blood mononuclear cells (PBMC) for diagnosis of pulmonary tuberculosis.
Polymerase chain reaction (PCR) technique was used to amplify gene of 240 bp DNA fragment prepared by Triton X-100 method, and some factors affecting PCR result were also analysed.
In blood samples of 89 patients with pulmonary tuberculosis and in sputum samples of 84 patients with pulmonary tuberculosis, the positive rates of PCR were 73% and 57% respectively, and the total positive rate of combinative detection of blood and sputum samples was 87% in 84 pulmonary tuberculosis patients. In 30 non-tuberculosis patients, 3 showed MTB-DNA positive.
PCR technique prepared by Triton X-100 method is rapid, simple, specific, and sensitive for detection of MTB-DNA in PBMC, and its sensitivity and accuracy could be increased in combination with sputum MTB examination. Detection of MTB-DNA in PMBC is of value in diagnosis of pulmonary tuberculosis.
评估检测外周血单个核细胞（PBMC）中结核分枝杆菌DNA（MTB-DNA）对肺结核诊断的临床价值。
采用聚合酶链反应（PCR）技术，通过Triton X-100法扩增制备的240 bp DNA片段基因，并分析影响PCR结果的一些因素。
89例肺结核患者血液样本及84例肺结核患者痰液样本中，PCR阳性率分别为73%和57%，84例肺结核患者血痰联合检测总阳性率为87%。30例非结核患者中，3例MTB-DNA呈阳性。
Triton X-100法制备的PCR技术检测PBMC中MTB-DNA快速、简便、特异、灵敏，与痰液MTB检测联合可提高其敏感性和准确性。检测PMBC中MTB-DNA对肺结核诊断有价值。
