El Din Awad O M, Abdel Hamid N F
Enzyme. 1975;20(4):221-33. doi: 10.1159/000458943.
The activities of alkaline phosphatases of kidney and urine appear similar and identical in action. Both are magnesium-dependent and inhibited by higher concentrations of 2-glycerophosphate. A band of similar mobility is obtained on electrophoresis of kidney and urine enzymes. In vitro magnesium competitively inhibits their activities when the ratio of Mg-+2 ions/2-glycerophosphate is above a definite level. Their activities are not affected by exogenous zinc and follow the Michaelis-Menten equation only when attention is given to the ratio of Mg-+2 ions/substrate. Nitrofuranfuradroxyl is a mixed type inhibitor showing a second-order rate of reaction with kidney and urine phosphatases.
肾脏和尿液中碱性磷酸酶的活性在作用上似乎相似且相同。两者都依赖镁离子，且会被高浓度的2-甘油磷酸酯抑制。对肾脏和尿液中的酶进行电泳时，会得到具有相似迁移率的条带。在体外，当镁离子与2-甘油磷酸酯的比例高于一定水平时，镁离子会竞争性抑制它们的活性。它们的活性不受外源锌的影响，并且只有在关注镁离子与底物的比例时才遵循米氏方程。硝呋烯腙是一种混合型抑制剂，与肾脏和尿液中的磷酸酶反应呈现二级反应速率。
