Cserháti T, Forgács E, Deyl Z, Miksik I, Eckhardt A
Institute of Chemistry, Chemical Research Centre, Hungarian Academy of Sciences, Budapest.
J Chromatogr A. 2001 Feb 23;910(1):137-45. doi: 10.1016/s0021-9673(00)01191-2.
The relative strength of interaction between anionic (SDS) and nonionic surfactant (octaethoxylated oleyl alcohol, GEN) and homologous series of peptides was determined by reversed-phase thin-layer chromatography (RP-TLC) carried out on alumina layers impregnated with paraffin oil. The relative strength of interaction was calculated and was correlated with the physicochemical parameters of peptides. It was established that each peptide interacted with both surfactants and with their mixture (1:1, m/m). The relative strength of interaction depended on the number of amino acid units in the peptide, side chain bulk and electronic properties and hydrophobicity of the amino acids. The impact of individual parameters highly depended on the character of surfactant. The data prove that the retention order of peptides can be modified by adding different surfactants and surfactant mixtures to the mobile phase resulting in improved separation.
通过在浸渍有石蜡油的氧化铝层上进行反相薄层色谱法（RP-TLC），测定了阴离子表面活性剂（SDS）与非离子表面活性剂（八乙氧基化油醇，GEN）以及同源肽系列之间相互作用的相对强度。计算了相互作用的相对强度，并将其与肽的物理化学参数相关联。结果表明，每种肽都与两种表面活性剂及其混合物（1:1，质量比）相互作用。相互作用的相对强度取决于肽中氨基酸单元的数量、氨基酸侧链的体积、电子性质和疏水性。各个参数的影响高度依赖于表面活性剂的性质。数据证明，通过向流动相中添加不同的表面活性剂和表面活性剂混合物，可以改变肽的保留顺序，从而改善分离效果。
