Lee W, Cho D, Chun B O, Chang T, Ree M
Department of Chemistry and Center for Integrated Molecular Systems, Pohang University of Science and Technology, South Korea.
J Chromatogr A. 2001 Feb 23;910(1):51-60. doi: 10.1016/s0021-9673(00)01163-8.
Temperature gradient interaction chromatography (TGIC) is applied to the characterization of polyisoprene (PI) and polystyrene (PS) using normal-phase (NP) stationary phase--bare silica or diol bonded silica. Tetrahydrofuran-isooctane mixtures are used as a mobile phase. PI and linear and star shaped PS samples are successfully fractionated in terms of the molecular mass with a high resolution comparable to that of reversed-phase (RP) HPLC. Temperature dependence of the retention shows that the enthalpy of adsorption of PS to the stationary phase is exothermic. In addition, some characteristic features of the NP-TGIC system relative to those of RP-TGIC are presented, which include a high sensitivity on the polar end group and the simultaneous size-exclusion chromatographic and TGIC characterization of PS and PI mixtures.
温度梯度相互作用色谱法（TGIC）用于使用正相（NP）固定相——裸硅胶或二醇键合硅胶对聚异戊二烯（PI）和聚苯乙烯（PS）进行表征。四氢呋喃 - 异辛烷混合物用作流动相。PI以及线性和星形PS样品成功地按分子量进行了分馏，分辨率高，可与反相（RP）高效液相色谱相媲美。保留的温度依赖性表明，PS在固定相上的吸附焓是放热的。此外，还介绍了NP - TGIC系统相对于RP - TGIC系统的一些特征，包括对极性端基的高灵敏度以及PS和PI混合物的同时尺寸排阻色谱和TGIC表征。
