Lorenz Myriam R, Kohnle Maria-Verena, Dass Martin, Walther Paul, Höcherl Anita, Ziener Ulrich, Landfester Katharina, Mailänder Volker
Institute of Organic Chemistry III - Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
Macromol Biosci. 2008 Aug 11;8(8):711-27. doi: 10.1002/mabi.200700336.
Fluorescent polyisoprene nanoparticles were synthesized by the miniemulsion technique as marker particles for cells. The uptake of the non-functionalized polyisoprene nanoparticles, without any transfection agents, into different adherent (HeLa) and also suspension (Jurkat) cell lines is strikingly efficient and fast compared to other polymeric particles, and leads to high loading of the cells. The intracellular polyisoprene particles are localized as single particles in endosomes distributed throughout the entire cytoplasm. The uptake kinetics shows that particle internalization starts during the first minutes of incubation and is finished after 48 h of incubation. Since (unfunctionalized) polystyrene particles show a comparable, low uptake behavior in cells, the uptake rates can be tuned by the amount of polystyrene in polyisoprene/polystyrene copolymer particles. As polyisoprene nanoparticles are internalized by different cell lines that are relevant for biomedical applications, they can be used to label these cells efficiently if a marker is incorporated in the particles. As polyisoprene is not or is hardly biodegradable the particles should be suited for long-term applications.
通过微乳液技术合成了荧光聚异戊二烯纳米颗粒，作为细胞的标记颗粒。与其他聚合物颗粒相比，未功能化的聚异戊二烯纳米颗粒在没有任何转染剂的情况下，进入不同的贴壁细胞系（HeLa）和悬浮细胞系（Jurkat）的效率惊人地高且速度快，并导致细胞的高负载量。细胞内的聚异戊二烯颗粒以单个颗粒的形式定位在内体中，分布于整个细胞质中。摄取动力学表明，颗粒内化在孵育的最初几分钟开始，并在孵育48小时后完成。由于（未功能化的）聚苯乙烯颗粒在细胞中表现出类似的低摄取行为，摄取速率可以通过聚异戊二烯/聚苯乙烯共聚物颗粒中聚苯乙烯的量来调节。由于聚异戊二烯纳米颗粒被与生物医学应用相关的不同细胞系内化，如果在颗粒中掺入标记物，它们可以有效地用于标记这些细胞。由于聚异戊二烯不易或几乎不可生物降解，这些颗粒应适用于长期应用。
