Papadimitriou E, Polykratis A, Courty J, Koolwijk P, Heroult M, Katsoris P
Laboratory of Molecular Pharmacology, University of Patras, Patras, Greece.
Biochem Biophys Res Commun. 2001 Mar 23;282(1):306-13. doi: 10.1006/bbrc.2001.4574.
HARP (heparin affin regulatory peptide) is a growth factor displaying high affinity for heparin. In the present work, we studied the ability of human recombinant HARP as well as its two terminal peptides (HARP residues 1-21 and residues 121-139) to promote angiogenesis. HARP stimulates endothelial cell tube formation on matrigel, collagen and fibrin gels, stimulates endothelial cell migration and induces angiogenesis in the in vivo chicken embryo chorioallantoic membrane assay. The two HARP peptides seem to be involved in most of the angiogenic effects of HARP. They both stimulate in vivo angiogenesis and in vitro endothelial cell migration and tube formation on matrigel. We conclude that HARP has an angiogenic activity when applied exogenously in several in vitro and in vivo models of angiogenesis and its NH(2) and COOH termini seem to play an important role.
肝素亲和调节肽（HARP）是一种对肝素具有高亲和力的生长因子。在本研究中，我们研究了重组人HARP及其两个末端肽（HARP的1 - 21位残基和121 - 139位残基）促进血管生成的能力。HARP可刺激内皮细胞在基质胶、胶原蛋白和纤维蛋白凝胶上形成管腔，刺激内皮细胞迁移，并在鸡胚绒毛尿囊膜体内试验中诱导血管生成。这两个HARP肽似乎参与了HARP的大部分血管生成作用。它们都能刺激体内血管生成以及体外内皮细胞迁移和在基质胶上形成管腔。我们得出结论，在几种体外和体内血管生成模型中外源应用时，HARP具有血管生成活性，其氨基和羧基末端似乎起着重要作用。
