一种用于药物渗透和毒性研究的简易角膜灌注室。
A simple corneal perfusion chamber for drug penetration and toxicity studies.
作者信息
Thiel M A, Morlet N, Schulz D, Edelhauser H F, Dart J K, Coster D J, Williams K A
机构信息
Department of Ophthalmology, Flinders University of South Australia, Adelaide, Australia.
出版信息
Br J Ophthalmol. 2001 Apr;85(4):450-3. doi: 10.1136/bjo.85.4.450.
AIMS
Corneal perfusion chambers are important tools in the development and assessment of ophthalmic drugs. The aim of this study was to design and test a modified perfusion chamber suitable for topical application of drugs to isolated corneoscleral preparations, and which allowed continuous monitoring of endothelial cell function.
METHODS
A polycarbonate and stainless steel perfusion chamber was designed to clamp corneas in a horizontal plane suitable for topical drug delivery. Endothelial cell function was assessed by ultrasonic pachymetry and specular microscopy during perfusion. Epithelial barrier function was assessed by penetration of fluorescein. Leakage was examined by measuring penetration of a large protein, IgG. Tissue architecture after perfusion was examined by conventional histology.
RESULTS
Corneas maintained a functionally and morphologically intact endothelial monolayer during perfusion periods of up to 14 hours. The epithelial barrier function was well preserved. The tissue clamp sealed the preparation effectively against leakage of macromolecules.
CONCLUSION
The new chamber device forms a reliable tool for in vitro drug penetration and toxicity studies in isolated perfused corneoscleral tissue.
目的
角膜灌注室是眼科药物研发和评估中的重要工具。本研究的目的是设计并测试一种改良的灌注室，该灌注室适用于将药物局部应用于分离的角膜巩膜制剂，并能持续监测内皮细胞功能。
方法
设计了一种聚碳酸酯和不锈钢灌注室，用于在适合局部给药的水平面上夹持角膜。在灌注过程中，通过超声测厚法和镜面显微镜评估内皮细胞功能。通过荧光素渗透评估上皮屏障功能。通过测量大分子IgG的渗透来检测渗漏情况。灌注后的组织结构通过传统组织学进行检查。
结果
在长达14小时的灌注期间，角膜维持了功能和形态完整的内皮单层。上皮屏障功能得到良好保存。组织夹有效地密封了制剂，防止大分子渗漏。
结论
新的室装置为在分离的灌注角膜巩膜组织中进行体外药物渗透和毒性研究提供了可靠的工具。
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