Blesa D, Gandía M, Martínez-Sebastián M J
Departament de Genètica, Universitat de València, València, Spain.
Mol Biol Evol. 2001 Apr;18(4):585-92. doi: 10.1093/oxfordjournals.molbev.a003839.
The bilbo element is a non-LTR retrotransposon isolated from Drosophila subobscura. We conducted a distribution survey by Southern blot for 52 species of the family Drosophilidae, mainly from the obscura and melanogaster groups. Most of the analyzed species bear sequences homologous to bilbo from D. subobscura. In the obscura group, species from the same species subgroup also share similar Southern blot patterns. To investigate the phylogenetic relationship among these elements, we analyzed eight copies of a short sequence of the element from several species of the obscura group. The obtained phylogram agrees with the phylogeny of the species, which suggests vertical transmission of the element.
比尔博元件是从果蝇中分离出的一种非长末端重复逆转座子。我们通过Southern杂交对比果蝇科的52个物种进行了分布调查，这些物种主要来自果蝇亚属和黑腹果蝇亚属。大多数分析的物种都含有与果蝇亚属的比尔博元件同源的序列。在果蝇亚属中，同一物种亚组的物种也具有相似的Southern杂交图谱。为了研究这些元件之间的系统发育关系，我们分析了果蝇亚属几个物种中该元件短序列的八个拷贝。得到的系统发育树与物种的系统发育一致，这表明该元件是垂直传递的。
