Blesa D, Martínez-Sebastián M J
Departamento de Genética, Universidad de Valencia, Spain.
Mol Biol Evol. 1997 Nov;14(11):1145-53. doi: 10.1093/oxfordjournals.molbev.a025724.
We used the repetitive character of transposable elements to isolate a non-LTR retrotransposon in Drosophila subobscura. bilbo, as we have called it, has homology to TRIM and LOA elements. Sequence analysis showed a 5' untranslated region (UTR), an open reading frame (ORF) with no RNA-binding domains, a downstream ORF that had structural homology to that of the I factor, and, finally, a 3' UTR which ended in several 5-nt repeats. The results of our phylogenetic and structural analyses shed light on the evolution of Drosophila non-LTR retrotransposons and support the hypothesis that an ancestor of these elements was structurally complex.
我们利用转座元件的重复特性，在果蝇中分离出一种非长末端重复序列（non-LTR）反转录转座子。我们将其命名为bilbo，它与TRIM和LOA元件具有同源性。序列分析显示，它有一个5'非翻译区（UTR）、一个没有RNA结合结构域的开放阅读框（ORF）、一个与I因子结构同源的下游ORF，最后还有一个以几个5核苷酸重复序列结尾的3'UTR。我们的系统发育和结构分析结果揭示了果蝇非LTR反转录转座子的进化，并支持了这些元件的祖先在结构上很复杂这一假说。
