精子染色质结构分析对生育力评估很有用。
Sperm chromatin structure assay is useful for fertility assessment.
作者信息
Evenson D, Jost L
机构信息
Department of Chemistry and Biochemistry, Box 2170 ASC 136, South Dakota State University, Brookings, South Dakota 57007-1217, USA.
出版信息
Methods Cell Sci. 2000;22(2-3):169-89. doi: 10.1023/a:1009844109023.
The Sperm Chromatin Structure Assay (SCSA) serves as a tool for measuring clinically important properties of sperm nuclear chromatin integrity. The assay utilizes the metachromatic features of Acridine Orange (AO), a DNA probe, and the principles of flow cytometry (FCM). SCSA data are not well correlated with classical sperm quality parameters and have been solidly shown to predict sub/infertility. This assay is ideally suited to human and animal fertility clinics to assess male sperm DNA integrity as related to fertility potential and embryo development as well as effects of reproductive toxicants. A detailed description of the SCSA follows.
精子染色质结构分析（SCSA）是一种用于测量精子核染色质完整性临床重要特性的工具。该分析利用了DNA探针吖啶橙（AO）的异染特性以及流式细胞术（FCM）的原理。SCSA数据与经典的精子质量参数相关性不佳，并且已确凿地证明可预测亚不育/不育情况。该分析非常适合人类和动物生育诊所，用于评估与生育潜力和胚胎发育相关的男性精子DNA完整性以及生殖毒物的影响。以下是SCSA的详细描述。
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