Gryczyński M, Papierz W, Niewiadomska H, Kobos J, Pietruszewska W
Katedra i Klinika Otolaryngologii AM w Łodzi.
Otolaryngol Pol. 2000;54(6):669-74.
One of the most important factor in prognosis of the patients with laryngeal cancer is presence of the metastases in lymph nodes of the neck. The main purpose of the paper was the evaluation of CD34 and FVIII antigens as angiogenesis markers, and nm23 protein and CD44 antigen expression as metastasis potential markers and description of their role in the tumour progression and making metastasis in the patients with laryngeal cancer. Paraffin-embedded tissue sections from 89 patients with laryngeal cancer were stained with a monoclonal antibody raised against CD34 and FVIII antigens, against nm 23 protein and against CD44 antigen. Measuring the density of the microvasculature in tumour was investigated. We found significant dependence between intensity of angiogenesis (IA) and pT, nodal metastasis, histological grading and survival. There were also significant correlation between nm23 protein expression and nodal metastasis, and between CD44 antigen expression and pT, nm23 protein expression and FVIII antigen expression. Evaluation of mentioned markers allowed to asses the aggressiveness of tumour cells and anticipate neck metastasis in the patients with laryngeal cancer.
喉癌患者预后的最重要因素之一是颈部淋巴结有无转移。本文的主要目的是评估作为血管生成标志物的CD34和FVIII抗原，以及作为转移潜能标志物的nm23蛋白和CD44抗原的表达，并描述它们在喉癌患者肿瘤进展和发生转移中的作用。对89例喉癌患者的石蜡包埋组织切片用针对CD34和FVIII抗原、nm23蛋白以及CD44抗原的单克隆抗体进行染色。研究了测量肿瘤微血管密度的情况。我们发现血管生成强度（IA）与pT、淋巴结转移、组织学分级和生存率之间存在显著相关性。nm23蛋白表达与淋巴结转移之间、CD44抗原表达与pT之间、nm23蛋白表达与FVIII抗原表达之间也存在显著相关性。对上述标志物的评估有助于评估肿瘤细胞的侵袭性并预测喉癌患者的颈部转移情况。
