Gryczyński M, Niewiadomska H, Kobos J, Pietruszewska W
Katedra i Klinika Otolaryngologii AM w Łodzi.
Otolaryngol Pol. 2000;54(6):675-9.
Evaluation of the biology of laryngeal cancer cell is connected either with many process inside the cell or reactions between cancer cell itself and extracellular matrix. The main purpose in this paper was the evaluation of p53 protein, bcl-2 protein, Ki-67 antigen and CD44 adhesive molecule expressions in comparison to clinical and histopathological features in patients with laryngeal cancer. Paraffin-embedded tissue sections from 89 patients with laryngeal cancer were stained with a monoclonal antibody raised against p53 and bcl-2 proteins, Ki-67 and CD44 antigens using a peroxidase-labelled streptavidin-biotin kit. There were statistically significant relationships between p-53 protein over-expression and pT, histological grading, survival and Ki-67 and CD44 antigens expressions. There were no correlation between bcl-2 protein expression and clinical and histopathological features. We observed statistically significant correlation between Ki-67 expression and pT, histological grading, recurrences and survival. Expression of CD44 statistically significant correlated only with tumour size. We conclude that comparison of data covering mentioned tumour markers expression gives valuable evaluation of biological activity of cancer cells and may allow to create the immunological panel of tumour markers which simplify the prognosis about nodal metastases, recurrences and survival in patients with laryngeal cancer.
喉癌细胞生物学特性的评估既与细胞内的许多过程有关，也与癌细胞自身和细胞外基质之间的反应有关。本文的主要目的是评估p53蛋白、bcl-2蛋白、Ki-67抗原和CD44黏附分子的表达，并与喉癌患者的临床和组织病理学特征进行比较。使用过氧化物酶标记的链霉亲和素-生物素试剂盒，用针对p53和bcl-2蛋白、Ki-67和CD44抗原的单克隆抗体对89例喉癌患者的石蜡包埋组织切片进行染色。p53蛋白过度表达与pT、组织学分级、生存率以及Ki-67和CD44抗原表达之间存在统计学上的显著关系。bcl-2蛋白表达与临床和组织病理学特征之间无相关性。我们观察到Ki-67表达与pT、组织学分级、复发和生存率之间存在统计学上的显著相关性。CD44的表达仅与肿瘤大小存在统计学上的显著相关性。我们得出结论，比较上述肿瘤标志物表达的数据可以对癌细胞的生物学活性进行有价值的评估，并可能有助于创建肿瘤标志物免疫组，从而简化对喉癌患者淋巴结转移、复发和生存率的预后判断。
