Lee J H, Seo J M, Kim M, Kim J C
Department of Immunology, Kyungpook National University School of Medicine, Deagu, Korea.
Mol Cells. 2001 Feb 28;11(1):100-4.
Using a low abundant gene screening strategy in the human dermal papilla cell cDNA library, we isolated a novel cDNA, which was 1,872 bp of nucleotides in length and contained an open reading frame encoding 405 amino acids. We designated it 'fibrinogen/angiopoietin-related protein' (FARP) as it contained the characteristic coiled-coil domain and fibrinogen-like domain in the NH2- and COOH-terminal, which are conserved in angiopoietins. FARP has a highly hydrophobic region at the N-terminus that is typical of a secretory signal sequence. Recently, a very similar gene, HFARP, was cloned and they have a difference of only 18 amino acids in N-terminus. While HFARP was expressed only in the liver, northern blot analysis showed that FARP mRNA is abundantly expressed in the liver, placenta, prostate, and ovary in human adult tissues. It was also expressed in the fetal liver and lung carcinoma cell line. Further study will be needed to clarify the function of the FARP gene.
我们在人真皮乳头细胞cDNA文库中采用低丰度基因筛选策略，分离出一个新的cDNA，其长度为1872个核苷酸，包含一个编码405个氨基酸的开放阅读框。我们将其命名为“纤维蛋白原/血管生成素相关蛋白”（FARP），因为它在氨基末端和羧基末端含有在血管生成素中保守的特征性卷曲螺旋结构域和纤维蛋白原样结构域。FARP在N端有一个高度疏水的区域，这是分泌信号序列的典型特征。最近，一个非常相似的基因HFARP被克隆出来，它们在N端仅相差18个氨基酸。虽然HFARP仅在肝脏中表达，但Northern印迹分析表明，FARP mRNA在成人组织的肝脏、胎盘、前列腺和卵巢中大量表达。它也在胎儿肝脏和肺癌细胞系中表达。需要进一步研究以阐明FARP基因的功能。
