Lee M E, Kweon S M, Ha K S, Nham S U
Division of Science Education, Kangwon National University, Choonchun, Korea.
Mol Cells. 2001 Feb 28;11(1):13-20.
Fibrin plays important roles in the wound healing processes, including blood clotting and platelet aggregation. Additional activities of fibrin were found in this study, which utilizes human THP-1 cells treated 1,25-(OH)2 vitamin D3 and plasminolytic fragments derived from fibrin. Coated fibrin fragment E on culture plates induced cell adhesions and morphological changes of the THP-1 cells, being resembled to tissue macrophages. Morphological changes of the THP-1 cells were caused by microfilament reorganization. IL-1beta production was increased in the THP-1 cells by adherent fibrin fragment E, but not by fibrin fragment D or by fibrinogen fragment E. The elevation of IL-1beta production is caused by transcriptional activation. Incubation with cytochalacin D, an actin polymerization inhibitor, prevents both microfilament reorganization and morphological changes, but has no effect on the IL-1beta production stimulated by fibrin fragment E. This data suggests that the IL-1beta production in the THP-1 cells do not require microfilament reorganization and integrin aggregation. Taken together, these results indicate that fibrin matrix plays an additional role in the stimulation of monocytes for production of IL-1beta, morphological changes and cell adhesion, resulting in the facilitation of the wound healing processes.
纤维蛋白在伤口愈合过程中发挥着重要作用，包括血液凝固和血小板聚集。本研究发现了纤维蛋白的其他活性，该研究利用经1,25-(OH)₂维生素D₃处理的人THP-1细胞以及源自纤维蛋白的纤溶片段。在培养板上包被的纤维蛋白片段E诱导了THP-1细胞的细胞黏附及形态变化，使其类似于组织巨噬细胞。THP-1细胞的形态变化是由微丝重组引起的。黏附的纤维蛋白片段E可使THP-1细胞中IL-1β的产生增加，但纤维蛋白片段D或纤维蛋白原片段E则无此作用。IL-1β产生的增加是由转录激活引起的。用肌动蛋白聚合抑制剂细胞松弛素D孵育可防止微丝重组和形态变化，但对纤维蛋白片段E刺激的IL-1β产生没有影响。该数据表明，THP-1细胞中IL-1β的产生不需要微丝重组和整合素聚集。综上所述，这些结果表明纤维蛋白基质在刺激单核细胞产生IL-1β、形态变化和细胞黏附方面发挥了额外作用，从而促进了伤口愈合过程。
