蚕豆萎蔫蚕豆病毒新加坡分离株的全序列及系统发育分析

Complete sequences and phylogenetic analyses of a Singapore isolate of broad bean wilt fabavirus.

作者信息

Koh L H, Cooper J I, Wong S M

机构信息

Department of Biological Sciences, The National University of Singapore.

出版信息

Arch Virol. 2001;146(1):135-47. doi: 10.1007/s007050170197.

DOI:10.1007/s007050170197

PMID:11266207

Abstract

The complete nucleotide (nt) sequence of a Singapore isolate of broad bean wilt fabavirus from Megakepasma erythrochlamys L., designated BBWV-ME, was determined. Its bipartite genome consisted of two positive-sense single-stranded ribonucleic acids (RNA). RNA1 (5951 nt in length) encoded a putative protease cofactor, nucleotide triphosphate (NTP)-binding domain (helicase), viral genome-linked protein (VPg), protease and RNA-dependent RNA polymerase (RdRp). RNA2 (3607 nt in length) encoded a putative movement protein (MP) and coat proteins (CP). Genome organization of BBWV-ME was similar to other viruses in the Comoviridae family. Phylogenetic analyses showed that fabaviruses were more closely related to the comoviruses than the nepoviruses.

摘要

测定了从红苞喜林芋（Megakepasma erythrochlamys L.）分离得到的一株新加坡蚕豆萎蔫蚕豆病毒（fabavirus）的完整核苷酸（nt）序列，命名为BBWV-ME。其双分体基因组由两条正链单链核糖核酸（RNA）组成。RNA1（长度为5951 nt）编码一个假定的蛋白酶辅因子、核苷酸三磷酸（NTP）结合结构域（解旋酶）、病毒基因组连接蛋白（VPg）、蛋白酶和RNA依赖的RNA聚合酶（RdRp）。RNA2（长度为3607 nt）编码一个假定的运动蛋白（MP）和外壳蛋白（CP）。BBWV-ME的基因组结构与豆病毒科的其他病毒相似。系统发育分析表明，蚕豆病毒与豇豆花叶病毒的亲缘关系比与线虫传多面体病毒更近。