Sadofsky M J
Medical College of Georgia, Institute of Molecular Medicine and Genetics, CB-2803, Augusta, GA 30912, USA.
Nucleic Acids Res. 2001 Apr 1;29(7):1399-409. doi: 10.1093/nar/29.7.1399.
V(D)J recombination is the process that generates the diversity among T cell receptors and is one of three mechanisms that contribute to the diversity of antibodies in the vertebrate immune system. The mechanism requires precise cutting of the DNA at segment boundaries followed by rejoining of particular pairs of the resulting termini. The imprecision of aspects of the joining reaction contributes significantly to increasing the variability of the resulting functional genes. Signal sequences target DNA recombination and must participate in a highly ordered protein-DNA complex in order to limit recombination to appropriate partners. Two proteins, RAG1 and RAG2, together form the nuclease that cleaves the DNA at the border of the signal sequences. Additional roles of these proteins in organizing the reaction complex for subsequent steps are explored.
V(D)J重排是产生T细胞受体多样性的过程，也是脊椎动物免疫系统中促成抗体多样性的三种机制之一。该机制需要在片段边界精确切割DNA，然后将特定的末端对重新连接。连接反应某些方面的不精确性对增加最终功能基因的变异性有显著贡献。信号序列靶向DNA重组，并且必须参与高度有序的蛋白质-DNA复合物，以便将重组限制在合适的伙伴之间。两种蛋白质RAG1和RAG2共同形成在信号序列边界切割DNA的核酸酶。还探讨了这些蛋白质在组织后续步骤反应复合物中的其他作用。
