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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Hughes G M, O'Neill J G, van Aardt W J

J Exp Biol. 1976 Aug;65(1):21-38. doi: 10.1242/jeb.65.1.21.

A detailed account is given of an electrolytic method for determining the oxygen dissociation curve of fish blood using a single sample of 50-100 mul for the whole curve. The accuracy and some of the problems arising from its uses are discussed. 2. Oxygen dissociation curves have been determined for trout blood and human blood at temperatures of 15 and 37 degrees C. The relationship between P50 and temperature is similar to that obtained using other methods. Absolute values of P50 are generally lower than those obtained by other methods, especially in the case of fish blood. 3. The effect of PCO2 and pH on the oxygen dissociation curve of trout blood is tested and it is shown that PCO2 has a more marked effect than pH when the other factor is maintained at a constant level. The Bohr factor (delta log P50/delta pH) appears to be approximately the same and independent of the PCO2. 4. The P50 of ray blood determined from fish during and after an operation showed an increased Bohr factor.

详细介绍了一种电解方法，该方法可使用50 - 100微升的单个样本测定鱼血的氧解离曲线，用于整个曲线的测定。讨论了该方法的准确性及其使用中出现的一些问题。2. 已测定了15℃和37℃时鳟鱼血液和人血的氧解离曲线。P50与温度之间的关系与使用其他方法得到的结果相似。P50的绝对值通常低于通过其他方法获得的值，尤其是在鱼血的情况下。3. 测试了PCO2和pH对鳟鱼血液氧解离曲线的影响，结果表明，当另一个因素保持在恒定水平时，PCO2的影响比pH更显著。玻尔因子（δlog P50/δpH）似乎大致相同，且与PCO2无关。4. 对手术期间和手术后的鱼类测定的鳐鱼血液的P50显示玻尔因子增加。

Hughes G M, O'Neill J G, van Aardt W J

J Exp Biol. 1976 Aug;65(1):21-38. doi: 10.1242/jeb.65.1.21.

A detailed account is given of an electrolytic method for determining the oxygen dissociation curve of fish blood using a single sample of 50-100 mul for the whole curve. The accuracy and some of the problems arising from its uses are discussed. 2. Oxygen dissociation curves have been determined for trout blood and human blood at temperatures of 15 and 37 degrees C. The relationship between P50 and temperature is similar to that obtained using other methods. Absolute values of P50 are generally lower than those obtained by other methods, especially in the case of fish blood. 3. The effect of PCO2 and pH on the oxygen dissociation curve of trout blood is tested and it is shown that PCO2 has a more marked effect than pH when the other factor is maintained at a constant level. The Bohr factor (delta log P50/delta pH) appears to be approximately the same and independent of the PCO2. 4. The P50 of ray blood determined from fish during and after an operation showed an increased Bohr factor.

详细介绍了一种电解方法，该方法可使用50 - 100微升的单个样本测定鱼血的氧解离曲线，用于整个曲线的测定。讨论了该方法的准确性及其使用中出现的一些问题。2. 已测定了15℃和37℃时鳟鱼血液和人血的氧解离曲线。P50与温度之间的关系与使用其他方法得到的结果相似。P50的绝对值通常低于通过其他方法获得的值，尤其是在鱼血的情况下。3. 测试了PCO2和pH对鳟鱼血液氧解离曲线的影响，结果表明，当另一个因素保持在恒定水平时，PCO2的影响比pH更显著。玻尔因子（δlog P50/δpH）似乎大致相同，且与PCO2无关。4. 对手术期间和手术后的鱼类测定的鳐鱼血液的P50显示玻尔因子增加。