人类神经钙蛋白δ基因（NCALD）的分子克隆、定位与特性分析
Molecular cloning, mapping and characterization of the human neurocalcin delta gene (NCALD).
作者信息
Wang W, Zhou Z, Zhao W, Huang Y, Tang R, Ying K, Xie Y, Mao Y
机构信息
The Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, PR China.
出版信息
Biochim Biophys Acta. 2001 Mar 19;1518(1-2):162-7. doi: 10.1016/s0167-4781(00)00290-6.
We identified a new human gene that encodes a cognate of the bovine neurocalcin delta from a human fetal brain cDNA library; hence we named it human neurocalcin delta (NCALD) gene. The deduced polypeptide product of the cDNA is 22 kDa in size, and its amino acid sequence is 100% and 99% identical to that of the bovine and chicken neurocalcin, respectively. Northern blots showed that the NCALD gene is more abundantly expressed in brain, testis, ovary and small intestine. Tissue in situ hybridization confirmed the existence of the NCALD mRNA in the adult human testis. Radiation hybrid panel mapping localized the gene to chromosome 8 between molecular markers D8S270 and D8S257.
我们从人胎儿脑cDNA文库中鉴定出一个新的人类基因，它编码牛神经钙蛋白δ的同源物；因此我们将其命名为人类神经钙蛋白δ（NCALD）基因。该cDNA推导的多肽产物大小为22 kDa，其氨基酸序列与牛和鸡神经钙蛋白的氨基酸序列分别有100%和99%的同源性。Northern印迹显示，NCALD基因在脑、睾丸、卵巢和小肠中表达更为丰富。组织原位杂交证实了成人睾丸中存在NCALD mRNA。辐射杂种板定位将该基因定位于分子标记D8S270和D8S257之间的8号染色体上。
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