Courtney C H, Zeng Q
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Box 100125, Gainesville, FL 32610-0125, USA.
Vet Parasitol. 2001 Apr 19;96(4):317-22. doi: 10.1016/s0304-4017(01)00374-0.
Sensitivity and specificity of four in-clinic heartworm antigen test kits, AbboScreen (Abbott Laboratories), Snap PF (IDEXX Laboratories), Solo Step (HESKA Corporation), Witness (Synbiotics Corporation) and two heartworm antigen microwell plate assays, DiroCHEK (Synbiotics) and PetChek PF (IDEXX) were compared in a blinded study using serum or plasma drawn from 237 random source dogs, including 140 with necropsy-confirmed, low worm burden infections (minimum 1 worm, maximum 10, mean 2.3, median 3) and 97 confirmed heartworm-free at necropsy. In general, microwell format tests were more sensitive than membrane format tests and tests using ELISA technology were more sensitive than tests using lateral flow immunochromatographic technology. Percent sensitivity and specificity, respectively, were PetChek PF 76 and 97, DiroCHEK 71 and 94, SNAP PF 67 and 98, Solo Step 60 and 98, and AbboScreen 52 and 96. The Witness test protocol was changed by the manufacturer midway through the study, and the newer version of this test kit arrived containing a package insert alerting the user to a change in procedure, which purportedly resulted in improved sensitivity. PetChek was significantly more sensitive than all other tests except DiroCHEK and the new version of Witness. DiroCHEK was significantly more sensitive than all tests except PetCheck, SNAP and the new version of Witness. Snap was more sensitive than AbboScreen and the old version of Witness. Differences in specificity were not significant (P>0.05).
在一项盲法研究中，对四种临床心丝虫抗原检测试剂盒（Abbott Laboratories公司的AbboScreen、IDEXX Laboratories公司的Snap PF、HESKA Corporation公司的Solo Step、Synbiotics Corporation公司的Witness）以及两种心丝虫抗原微孔板检测方法（Synbiotics公司的DiroCHEK和IDEXX公司的PetChek PF）的敏感性和特异性进行了比较。该研究使用了从237只随机来源犬采集的血清或血浆，其中包括140只经尸检确认有低虫负荷感染的心丝虫病犬（最少1条虫，最多10条虫，平均2.3条，中位数3条）和97只经尸检确认无心丝虫感染的犬。总体而言，微孔板形式的检测比膜形式的检测更敏感，使用酶联免疫吸附测定（ELISA）技术的检测比使用侧向流动免疫层析技术的检测更敏感。PetChek PF的敏感性和特异性百分比分别为76%和97%，DiroCHEK为71%和94%，SNAP PF为67%和98%，Solo Step为60%和98%，AbboScreen为52%和96%。在研究进行到一半时，Witness检测试剂盒的制造商更改了检测方案，新版本的试剂盒随附的包装说明书提醒用户检测程序有变化，据称这提高了敏感性。PetChek比除DiroCHEK和新版本的Witness之外的所有其他检测都显著更敏感。DiroCHEK比除PetCheck、SNAP和新版本的Witness之外的所有检测都显著更敏感。SNAP比AbboScreen和旧版本的Witness更敏感。特异性方面的差异不显著（P>0.05）。
