用于链霉菌的改良木糖操纵子的xylE和xylTE报告基因：xylT效应分析

Modified xylE and xylTE reporter genes for use in Streptomyces: analysis of the effect of xylT.

作者信息

González-Cerón G, Licona P, Servín-González L

机构信息

Institute of Biomedical Research, National Autonomous University of Mexico, Apartado Postal 70228, Ciudad Universitaria, D.F., 04510 Mexico, Mexico.

出版信息

FEMS Microbiol Lett. 2001 Mar 15;196(2):229-34. doi: 10.1111/j.1574-6968.2001.tb10569.x.

DOI:10.1111/j.1574-6968.2001.tb10569.x

PMID:11267784

Abstract

The reporter gene xylE (encoding catechol 2,3-dioxygenase) has been modified for a more rational use in Streptomyces. Two reporter fragments, one containing xylE, and the other containing also the upstream gene xylT (which encodes a soluble ferredoxin), have been constructed to allow precise fusion of regulatory regions to the reporter genes. Identical fusions of these xylE and xylTE reporter fragments to the Streptomyces dagA and tipA promoters, in low and high copy number plasmids, show that the levels of xylE mRNA and catechol 2,3-dioxygenase activities are significantly higher when xylT is present.

摘要

报告基因xylE（编码儿茶酚2,3-双加氧酶）已被改造，以便在链霉菌中更合理地使用。构建了两个报告片段，一个包含xylE，另一个还包含上游基因xylT（编码一种可溶性铁氧化还原蛋白），以实现调控区域与报告基因的精确融合。在低拷贝数和高拷贝数质粒中，将这些xylE和xylTE报告片段与链霉菌dagA和tipA启动子进行相同的融合，结果表明，当存在xylT时，xylE mRNA水平和儿茶酚2,3-双加氧酶活性显著更高。