包含报告基因xylE的克隆盒。

Cloning cassettes containing the reporter gene xylE.

作者信息

Clark E, Cirvilleri G

机构信息

Department of Environmental Science, Policy, and Management, University of California, Berkeley 94720.

出版信息

Gene. 1994 Dec 30;151(1-2):329-30. doi: 10.1016/0378-1119(94)90680-7.

DOI:10.1016/0378-1119(94)90680-7

PMID:7828900

Abstract

Two pUC-derived vectors containing the promoterless xylE gene (encoding catechol 2,3-dioxygenase) of Pseudomonas putida mt-2 were constructed. The t(o) transcriptional terminator of phage lambda was placed downstream from the stop codon of xylE. The new vectors, pXT1 and pXT2, contain xylE and the t(o) terminator within a cloning cassette which can be excised with several endonucleases. When inserted into a transcribed sequence, this xylE cassette reports promoter activity and interrupts downstream transcription of the target sequence.

摘要

构建了两个源自pUC的载体，其包含恶臭假单胞菌mt-2无启动子的xylE基因（编码儿茶酚2,3-双加氧酶）。噬菌体λ的t(o)转录终止子置于xylE终止密码子的下游。新载体pXT1和pXT2在一个可被多种核酸内切酶切除的克隆盒内包含xylE和t(o)终止子。当插入转录序列时，这个xylE盒报告启动子活性并中断靶序列的下游转录。