Peixoto J C, Bando S Y, Ordoñez J A, Botelho B A, Trabulsi L R, Moreira-Filho C A
Departamento de Immunologia, Instituto de Ciências Biomédicus da Universidade São Paolo, SP, Brazil.
FEMS Microbiol Lett. 2001 Mar 15;196(2):239-44. doi: 10.1111/j.1574-6968.2001.tb10571.x.
Genomic diversity among 34 strains of Escherichia coli belonging to different serotypes of the O26 serogroup -- encompassing strains from different geographical origins and Shiga toxin-negative Brazilian strains -- was evaluated through random amplified polymorphic DNA (RAPD) analysis. Our results indicate that Brazilian and non-Brazilian O26 strains fall under distinct but closely related differentiation clusters. RFLP-PCR analysis of the fliC gene sequence was done in order to identify the H(-) serotypes and served to confirm the clustering pattern obtained in the dendrogram generated from RAPD data. The epidemiological significance of these data is discussed.
通过随机扩增多态性DNA（RAPD）分析，评估了34株属于O26血清群不同血清型的大肠杆菌的基因组多样性，这些菌株包括来自不同地理来源的菌株和巴西的志贺毒素阴性菌株。我们的结果表明，巴西和非巴西的O26菌株属于不同但密切相关的分化簇。对fliC基因序列进行了RFLP-PCR分析，以鉴定H(-)血清型，并用于确认从RAPD数据生成的树状图中获得的聚类模式。讨论了这些数据的流行病学意义。
