大肠杆菌O26:H11的分子血清分型
Molecular serotyping of Escherichia coli O26:H11.
作者信息
Durso Lisa M, Bono James L, Keen James E
机构信息
U.S. Meat Animal Research Center, U.S. Department of Agriculture/ARS, P.O. Box 166, State Spur 18D, Clay Center, NE 68933, USA.
出版信息
Appl Environ Microbiol. 2005 Aug;71(8):4941-4. doi: 10.1128/AEM.71.8.4941-4944.2005.
Abstract
Serotyping is the foundation of pathogenic Escherichia coli diagnostics; however, few laboratories have this capacity. We developed a molecular serotyping protocol that targets, genetically, the same somatic and flagellar antigens of E. coli O26:H11 used in traditional serotyping. It correctly serotypes strains untypeable by traditional methods, affording primary laboratories serotyping capabilities.
摘要
血清分型是致病性大肠杆菌诊断的基础；然而，具备这种能力的实验室很少。我们开发了一种分子血清分型方案，从基因层面针对传统血清分型中使用的大肠杆菌O26:H11的相同菌体抗原和鞭毛抗原。它能够正确地对传统方法无法分型的菌株进行血清分型，使基层实验室具备血清分型能力。
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