El-Alamy M A, Foote R H
Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
Anim Reprod Sci. 2001 Mar 30;65(3-4):245-54. doi: 10.1016/s0378-4320(00)00230-x.
Ten semen extenders were tested in two experiments for cryopreservation of semen collected from four Finn and four Dorset rams. Two ejaculates of semen were combined from each ram for testing each extender treatment. The extenders consisted of a series of commonly used egg yolk-TRIS media with and without sodium and triethanolamine lauryl sulfate (STLS), a similar extender with 3-N-morpholino propane sulfonic acid (MOPS), and milk and whey extenders. In Experiment 1, extender treatments were replicated with three sets of collections from the eight rams, and in Experiment 2 with two sets. The egg yolk-TRIS-glycerol-STLS (EY(1)TSTLS) extender was significantly superior to other extenders except whole milk in protecting the sperm during freezing and thawing. In Experiment 1, a 20% egg yolk-TRIS-glycerol-STLS extender preserved 71% of the progressively motile Finn sperm (post-thaw divided by pre-freeze percentage of motile sperm), and 76% of the Dorset sperm. In Experiment 2, the corresponding values for the same EY(1)TSTLS extender used with Finn and Dorset sperm were 86 and 64%, respectively. Without STLS the egg yolk extenders were significantly less effective in protecting cryopreserved ram sperm. This egg yolk-TRIS extender, containing STLS and glycerol, may hold promise for freezing ram sperm that could be used successfully for intracervical insemination.
在两项实验中，对10种精液稀释剂进行了测试，用于冷冻保存从4只芬兰公羊和4只多塞特公羊采集的精液。从每只公羊采集两份射精精液合并起来，用于测试每种稀释剂处理。这些稀释剂包括一系列常用的含或不含钠和月桂醇硫酸三乙醇胺（STLS）的蛋黄-TRIS培养基、一种含3-N-吗啉丙烷磺酸（MOPS）的类似稀释剂，以及牛奶和乳清稀释剂。在实验1中，对8只公羊的三组采集精液重复进行稀释剂处理，在实验2中重复进行两组。蛋黄-TRIS-甘油-STLS（EY(1)TSTLS）稀释剂在冷冻和解冻过程中保护精子方面，除全脂牛奶外，明显优于其他稀释剂。在实验1中，20%的蛋黄-TRIS-甘油-STLS稀释剂保存了71%的芬兰公羊精子的渐进性活动能力（解冻后活动精子百分比除以冷冻前百分比）以及76%的多塞特公羊精子的渐进性活动能力。在实验2中，用于芬兰公羊和多塞特公羊精子的相同EY(1)TSTLS稀释剂的相应值分别为86%和64%。不含STLS的蛋黄稀释剂在保护冷冻保存的公羊精子方面效果明显较差。这种含有STLS和甘油的蛋黄-TRIS稀释剂，可能有望用于冷冻公羊精子，从而成功用于子宫颈内授精。
