Inaba H, Ohmae H, Kano S, Faarado L, Boaz L, Leafasia J, Suzuki M
Department of Parasitology, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, Japan.
Parasitol Int. 2001 Mar;50(1):9-13. doi: 10.1016/s1383-5769(00)00066-0.
A study on chloroquine resistance of falciparum malaria was conducted in the Solomon Islands. Both in vitro and clinical tests were performed. In our regular studies of in vitro chloroquine susceptibility tests on Plasmodium falciparum from non-immuners in Japan, the threshold point to differentiate resistant and susceptible isolates was set at a 0. 114 microM chloroquine in the semi-micro culture system, and this point was also applicable in the study of the malaria parasite taken in the highly endemic malarious area with good coincidence with clinical observation. Variation in the incubation time (24-63) to reach the schizont stage of the isolated parasites were noted. It appeared that chloroquine resistant P. falciparum showed traits to reach the schizont stage within a shorter incubation period.
在所罗门群岛开展了一项关于恶性疟原虫氯喹耐药性的研究。进行了体外试验和临床试验。在我们对来自日本非免疫者的恶性疟原虫进行体外氯喹敏感性常规研究中，在半微量培养系统中，区分耐药和敏感分离株的阈值设定为0.114微摩尔氯喹，这一阈值在高度流行疟疾地区采集的疟原虫研究中也适用，且与临床观察结果高度吻合。观察到分离出的疟原虫达到裂殖体阶段的孵育时间（24 - 63小时）存在差异。似乎氯喹耐药的恶性疟原虫表现出在较短孵育期内达到裂殖体阶段的特征。
