van Dongen B E, Schouten S, Damsté J S
Department of Marine Biogeochemistry and Toxicology, Netherlands Institute for Sea Research (NIOZ), PO Box 59, 1790 AB Den Burg, Texel, The Netherlands.
Rapid Commun Mass Spectrom. 2001;15(7):496-500. doi: 10.1002/rcm.259.
Monosaccharides were derivatized using methylboronic acid and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), and the delta13C values of these derivatives measured by gas chromatography/combustion/isotope-ratio-monitoring mass spectrometry to determine the original 13C-content of the monosaccharides. Comparison with the measured off-line delta13 values of the monosaccharides shows that no fractionation in 13C takes place during derivatization. The methylboronic derivatization method has proven to be a new method for natural abundance isotopic analysis of intact monosaccharides (arabinose, xylose, fucose, fructose and glucose). The method is rapid, does not involve isotopic fractionation during derivatization, and gives more precise delta13C values than other methods reported. The method was successfully applied to determine the delta13C value of glucose of the freshwater alga Scenedesmus communis.
单糖用甲基硼酸和N,O-双(三甲基硅基)三氟乙酰胺(BSTFA)进行衍生化，通过气相色谱/燃烧/同位素比率监测质谱法测量这些衍生物的δ13C值，以确定单糖的原始13C含量。与单糖离线测量的δ13值比较表明，衍生化过程中没有发生13C分馏。甲基硼酸衍生化方法已被证明是一种用于完整单糖(阿拉伯糖、木糖、岩藻糖、果糖和葡萄糖)天然丰度同位素分析的新方法。该方法快速，衍生化过程中不涉及同位素分馏，并且比报道的其他方法给出更精确的δ13C值。该方法成功应用于测定淡水绿藻普通栅藻葡萄糖的δ13C值。
