Holzmann K, Klump B, Weis-Klemm M, Hsieh C J, Borchard F, Gregor M, Porschen R
Department of Gastroenterology, University of Tuebingen, Germany.
Anticancer Res. 2000 Sep-Oct;20(5C):3951-5.
Telomerase activity is frequently associated with neoplasia. It is a ribonucleoprotein capable of replacing telomeric DNA sequences that are lost at each cell division. Neoplastic progression in chronic ulcerative colitis is characterized by the development of epithelial dysplasia which is accompanied by genetic alterations. Therefore we tested telomerase activity in 128 biopsy samples of four colectomy specimens with long-standing ulcerative pancolitis by using the Telomerase PCR ELISA System. In three patients with multiple dysplastic or carcinomatous lesions, telomerase activity was detected in 22 samples with a regional association to dysplastic or carcinomatous areas. 15 of the samples with telomerase activity (68%) were found in dysplastic/carcinomatous samples or in the direct vicinity of dysplastic areas, 4 (18%), 2 positions (about 4 cm) and the remaining three (14%) not more than 3 positions away from such areas. In the fourth patient, resected because of clinical deterioration despite medical treatment and who had no dysplastic lesions, no telomerase activity was detected. These results show that telomerase activity might be used as a complementary marker to histology for the identification of patients with ulcerative colitis who are at an increased risk for neoplastic progression.
端粒酶活性常与肿瘤形成相关。它是一种核糖核蛋白，能够替代在每次细胞分裂时丢失的端粒DNA序列。慢性溃疡性结肠炎的肿瘤进展以伴有基因改变的上皮发育异常为特征。因此，我们使用端粒酶PCR ELISA系统检测了128份来自4例患有长期全结肠炎的结肠切除标本的活检样本中的端粒酶活性。在3例有多处发育异常或癌性病变的患者中，在22个样本中检测到端粒酶活性，这些样本与发育异常或癌性区域存在局部关联。15份具有端粒酶活性的样本（68%）见于发育异常/癌性样本或发育异常区域的紧邻部位，4份（18%）距离发育异常区域2个位置（约4厘米），其余3份（14%）距离此类区域不超过3个位置。在第4例患者中，尽管接受了药物治疗但因临床病情恶化而接受手术切除，该患者无发育异常病变，未检测到端粒酶活性。这些结果表明，端粒酶活性可作为一种辅助标志物，与组织学检查相结合，用于识别溃疡性结肠炎患者中肿瘤进展风险增加的患者。
