Løvig Tone, Andersen Solveig Norheim, Clausen Ole Petter, Rognum Torleiv Ole
Institute of Forensic Medicine, Department of Pathology, Akershus University Hospital HF, Lørenskog, Norway.
Scand J Gastroenterol. 2007 May;42(5):586-91. doi: 10.1080/00365520601013747.
Ulcerative colitis (UC) is a chronic inflammatory disease of the colon associated with a high risk of developing colorectal cancer. Several genetic alterations have been documented in dysplasia and cancer developing in UC. Concerning microsatellite instability (MSI), many contradictory results have been published. We therefore analysed a large, well-characterized UC material for MSI to elucidate its significance in long-standing UC.
From 33 patients, a total of 159 microdissected lesions and 165 mucosa samples obtained adjacent to the tissue blocks were analysed for MSI using the five standard markers recommended by the National Cancer Institute; D2S123, D5S346, D17S250, BAT-25 and BAT-26. In addition, 12 of the patients were investigated by a mini-satellite marker at the D1S7 locus.
High-level MSI (MSI-H) was detected in one villous adenoma with high-grade dysplasia and right-sided location. This represents 3.6% (1/28) of dysplastic mucosa investigated. No other lesions showed MSI in the five standard markers or at the D1S7 locus.
This study suggests that MSI is rare in UC-related neoplasia as well as non-neoplastic lesions, and does not contribute to the development of dysplasia.
溃疡性结肠炎（UC）是一种结肠慢性炎症性疾病，与结直肠癌发生的高风险相关。在UC中发生的发育异常和癌症中已记录了几种基因改变。关于微卫星不稳定性（MSI），已发表了许多相互矛盾的结果。因此，我们分析了大量特征明确的UC材料中的MSI，以阐明其在长期UC中的意义。
从33例患者中，使用美国国立癌症研究所推荐的5种标准标志物（D2S123、D5S346、D17S250、BAT-25和BAT-26）对总共159个显微切割病变和从组织块旁获取的165个黏膜样本进行MSI分析。此外，对12例患者使用D1S7位点的一个小卫星标志物进行研究。
在1例具有高级别发育异常且位于右侧的绒毛状腺瘤中检测到高水平MSI（MSI-H）。这占所研究的发育异常黏膜的3.6%（1/28）。在5种标准标志物或D1S7位点上，没有其他病变显示MSI。
本研究表明，MSI在UC相关肿瘤以及非肿瘤性病变中罕见，且对发育异常的发生没有作用。
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