Mazzini G, Bottiroli G, Prenna G
Histochem J. 1975 May;7(3):291-7. doi: 10.1007/BF01003598.
An electronic device is described for the automatic correction of fluorescence emission spectra obtained by digital microspectrofluorometry based on multichannel scaling and single photon detection as described previously. This device consists of: (a) an arithmetic unit for the correction of the spectral values and for the curve integration processes; (b) a circuit that operates directly on data in the memory of the multi-channel analyser by subtracting from then a pre-established value corresponding to the background; and (c) an averaging unit for calculating a mean for the spectral value.