Mazzini G, Bottiroli G, Prenna G
Histochem J. 1975 May;7(3):291-7. doi: 10.1007/BF01003598.
An electronic device is described for the automatic correction of fluorescence emission spectra obtained by digital microspectrofluorometry based on multichannel scaling and single photon detection as described previously. This device consists of: (a) an arithmetic unit for the correction of the spectral values and for the curve integration processes; (b) a circuit that operates directly on data in the memory of the multi-channel analyser by subtracting from then a pre-established value corresponding to the background; and (c) an averaging unit for calculating a mean for the spectral value.
描述了一种电子设备,用于对如前所述的基于多通道缩放和单光子检测的数字显微分光荧光法获得的荧光发射光谱进行自动校正。该设备包括:(a) 用于校正光谱值和进行曲线积分过程的算术单元;(b) 一种电路,通过从多通道分析仪的存储器中的数据中减去对应于背景的预先设定值来直接对数据进行操作;以及 (c) 用于计算光谱值平均值的平均单元。