荧光偏振：使用光子计数光度计进行测量
Fluorescence polarization: measurements with a photon-counting photometer.
作者信息
Jameson D M, Weber G, Spencer R D, Mitchell G
机构信息
Biochemistry Department, University of Illinois, Urbana, IL 61801, USA.
出版信息
Rev Sci Instrum. 1978 Apr;49(4):510. doi: 10.1063/1.1135451.
PMID:18699136
Abstract
A T-format, photon-counting polarization photometer was constructed to facilitate precise polarization measurements at low signal-to-noise ratios. The instrument optics and electronics are described. Several examples of the instrument's performance are given, including measurements on picomolar fluorescein solutions at low excitation resolution and the excitation polarization spectrum of indole (2 x 10(-4)M) at a resolution of 5 A.
摘要
构建了一种T型光子计数偏振光度计，以利于在低信噪比下进行精确的偏振测量。文中描述了该仪器的光学和电子元件。给出了该仪器性能的几个实例，包括在低激发分辨率下对皮摩尔荧光素溶液的测量以及在5埃分辨率下对吲哚（2×10⁻⁴M）的激发偏振光谱测量。
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