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多核苷酸磷酸化酶的新型单功能底物。2'（3'）-O-二氢肉桂酰核苷5'-二磷酸对引物寡核苷酸的“单次添加”。

Novel monofunctional substrates of polynucleotide phosphorylase. The "single-addition" of 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide.

作者信息

Kikuchi Y, Hirai K, Sakaguchi K

出版信息

J Biochem. 1975 Feb;77(2):469-72. doi: 10.1093/oxfordjournals.jbchem.a130747.

DOI:10.1093/oxfordjournals.jbchem.a130747
PMID:1126926
Abstract

A method was developed for stepwise wynthesis of oligonucleotides of difined wequence using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates as substrates for polynucleotide phosphorylase [ED 2.7.7.8]. Polynucleotide phosphorylase from Thermus thermophilus catalyzed the transfer of one 2'(3')-blocked ADP to the 3'-terminus of the primer trinucleoside diphosphate, ApApA. The product was 2'(3')-substituted triadenylyladenosine. The blocking group, dihydrocinnamoyl, could be removed completely from the product without destruction of the phosphodiester bond using alpha-chymotrypsin [ED 3.4.21.1] at neutral pH.

摘要

开发了一种使用2'（3'）-O-二氢肉桂酰核苷5'-二磷酸作为多核苷酸磷酸化酶[EC 2.7.7.8]的底物逐步合成特定序列寡核苷酸的方法。嗜热栖热菌的多核苷酸磷酸化酶催化一个2'（3'）-封闭的ADP转移到引物三磷酸腺苷二磷酸ApApA的3'-末端。产物是2'（3'）-取代的三腺苷酰腺苷。在中性pH条件下，使用α-胰凝乳蛋白酶[EC 3.4.21.1]可以从产物中完全去除封闭基团二氢肉桂酰，而不会破坏磷酸二酯键。

相似文献

1
Novel monofunctional substrates of polynucleotide phosphorylase. The "single-addition" of 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide.多核苷酸磷酸化酶的新型单功能底物。2'（3'）-O-二氢肉桂酰核苷5'-二磷酸对引物寡核苷酸的“单次添加”。
J Biochem. 1975 Feb;77(2):469-72. doi: 10.1093/oxfordjournals.jbchem.a130747.
2
Enzymatic synthesis of oligonucleotides of defined sequence. The "single addition" of 2(3)-O-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase.特定序列寡核苷酸的酶促合成。嗜热多核苷酸磷酸化酶催化2(3)-O-二氢肉桂酰核苷5'-二磷酸向引物寡核苷酸的“单次添加”。
J Biochem. 1976 Jul;80(1):33-7. doi: 10.1093/oxfordjournals.jbchem.a131254.
3
A method for the synthesis of oligonucleotide by single addition of 2'-O-(o-nitrobenzyl)nucleoside 5'-diphosphates using polynucleotide phosphorylase.一种使用多核苷酸磷酸化酶通过单次添加2'-O-(邻硝基苄基)核苷5'-二磷酸来合成寡核苷酸的方法。
Biochim Biophys Acta. 1979 Nov 22;565(1):192-8. doi: 10.1016/0005-2787(79)90095-9.
4
2'-O-(alpha-methoxyethyl)nucleoside 5'-diphosphates as "single-addition" substrates in the synthesis of specific oligoribonucleotides with polynucleotide phosphorylase.2'-O-(α-甲氧基乙基)核苷5'-二磷酸作为“单加成”底物用于用多核苷酸磷酸化酶合成特定的寡核糖核苷酸。
Biochemistry. 1973 Sep 25;12(20):3956-62. doi: 10.1021/bi00744a027.
5
"Single Addition" substrates for the synthesis of specific oligoribonucleotides with polynucleotide phosphorylase. Synthesis of 2'-(alpha-methoxyethy) nucleoside 5'-diphosphates.用于通过多核苷酸磷酸化酶合成特定寡核糖核苷酸的“单次添加”底物。2'-（α-甲氧基乙基）核苷5'-二磷酸的合成。
Biochemistry. 1975 Jul 15;14(14):3152-8. doi: 10.1021/bi00685a018.
6
An efficient synthesis of polyguanylic acid by a thermophilic polynucleotide phosphorylase.嗜热多核苷酸磷酸化酶高效合成聚鸟苷酸。
Biochim Biophys Acta. 1977 Jun 17;476(4):287-94. doi: 10.1016/0005-2787(77)90293-3.
7
Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme.特定序列脱氧核糖寡核苷酸的酶促合成。该酶的性质。
Nucleic Acids Res. 1974 Dec;1(12):1631-47. doi: 10.1093/nar/1.12.1631.
8
Addition of short guanylyl blocks to oligonucleotide primers with a thermophilic polynucleotide phosphorylase. Its application to the synthesis of oligonucleotides containing guanylyl residues.利用嗜热多核苷酸磷酸化酶向寡核苷酸引物添加短鸟苷酸片段及其在含鸟苷酸残基寡核苷酸合成中的应用
J Biochem. 1979 Nov;86(5):1427-32. doi: 10.1093/oxfordjournals.jbchem.a132660.
9
[Isolation and some properties of polynucleotide phosphorylase from E. coli].[大肠杆菌多核苷酸磷酸化酶的分离及某些性质]
Biokhimiia. 1981 May;46(5):802-8.
10
Thermophilic polynucleotide phosphorylase from Thermus thermophilus. Purification and properties of an altered form of enzyme which lacks phosphorolytic activity to polynycleotide.嗜热栖热菌的嗜热多核苷酸磷酸化酶。缺乏对多核苷酸磷酸解活性的酶变体形式的纯化及性质
Eur J Biochem. 1977 Aug 1;77(3):575-83. doi: 10.1111/j.1432-1033.1977.tb11701.x.

引用本文的文献

1
Polynucleotides. XLII1. Limited addition of 2'O-onitrobenzyl nucleotides to the 3'-end of ribooligonucleotide with polynucleotide phosphorylase.多核苷酸。XLII1。用多核苷酸磷酸化酶将2'-邻硝基苄基核苷酸有限地添加到核糖寡核苷酸的3'末端。
Nucleic Acids Res. 1976 Nov;3(11):3203-11. doi: 10.1093/nar/3.11.3203.
2
Enzymatic synthesis of a segment of bacteriophage Qbeta coat protein gene.噬菌体Qβ外壳蛋白基因一段序列的酶促合成
Nucleic Acids Res. 1978 Feb;5(2):591-8. doi: 10.1093/nar/5.2.591.
3
Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase.
用T4 RNA连接酶将单核苷酸添加到寡核糖核苷酸受体上。
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1270-3. doi: 10.1073/pnas.75.3.1270.