多核苷酸。XLII1。用多核苷酸磷酸化酶将2'-邻硝基苄基核苷酸有限地添加到核糖寡核苷酸的3'末端。
Polynucleotides. XLII1. Limited addition of 2'O-onitrobenzyl nucleotides to the 3'-end of ribooligonucleotide with polynucleotide phosphorylase.
作者信息
Ikehara M, Tanaka S, Fukui T, Ohtsuka E
出版信息
Nucleic Acids Res. 1976 Nov;3(11):3203-11. doi: 10.1093/nar/3.11.3203.
Abstract
2'-O-o-Nitrobenzyluridine, -cytidine and -adenosine were phosphorylated with phosphoryl chloride to the corresponding 5'-phosphates and led to 5'-diphosphates by the method of Moffatt and Khorana. These 2'-O-oNB-nucleoside 5'-diphosphates were incubated with a primer CpApA and polynucleotide phosphorylase in the presence of Mn2+. Tetranucleotides CpApApU, CpApApC and CpApApA were obtained after photosensitive removal of oNB groups in yields of 54-70%.
摘要
2'-O-邻硝基苄基尿苷、胞苷和腺苷用氯化磷酰磷酸化得到相应的5'-磷酸酯，再通过莫法特和霍拉纳的方法得到5'-二磷酸酯。这些2'-O-邻硝基苄基核苷5'-二磷酸酯在Mn2+存在下与引物CpApA和多核苷酸磷酸化酶一起温育。在光敏去除邻硝基苄基基团后，得到四核苷酸CpApApU、CpApApC和CpApApA，产率为54-70%。
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