Fukushima M, Ohashi T, Kojima M, Ohba K, Shimizu H, Sonoyama K, Nakano M
Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Lipids. 2001 Feb;36(2):129-34. doi: 10.1007/s11745-001-0698-4.
The effects of resistant starches of beans on serum cholesterol and hepatic low density lipoprotein (LDL) receptor mRNA in rats were investigated. Rats were fed a cholesterol-free diet with 150 g/kg corn starch (CS), 150 g/kg adzuki (Vigna angularis) starch (AS), 150 g/kg kintoki (Phaseolus vulgaris, variety) starch (KS), or 150 g/kg tebou (P. vulgaris, variety) starch (TS) for 4 wk. There were no significant differences in body weight among groups through the experimental period. The liver weight in the CS group was 1.1-1.2 times higher than that in the AS, KS, and TS groups. The cecum weight in the TS was 1.4 times higher than that in the CS group, and the cecal pH in the CS group was significantly higher than in the other groups. The serum total cholesterol, very low density lipoprotein + intermediate density lipoprotein + LDL-cholesterol and high density lipoprotein (HDL)-cholesterol concentrations in the bean starch groups were significantly lower than those in the CS group through the feeding period. The total cholesterol/HDL-cholesterol ratio in the bean starch groups was also significantly lower than that in the CS group at the end of the 4-wk feeding period. The hepatic cholesterol concentration in the TS group was significantly higher than in the CS group at the end of the 4-wk feeding period. The relative quantity of hepatic apo B mRNA in the AS group was 1.2 times higher than that in the CS group, and the hepatic LDL receptor mRNA levels in the AS and TS groups were 1.8-2.0 times higher than that in the CS group. The results of this study demonstrate that AS, KS, and TS lowered the serum total cholesterol level by enhancing the hepatic LDL receptor mRNA level.
研究了豆类抗性淀粉对大鼠血清胆固醇及肝脏低密度脂蛋白（LDL）受体mRNA的影响。将大鼠分为四组，分别喂食含150 g/kg玉米淀粉（CS）、150 g/kg赤豆（Vigna angularis）淀粉（AS）、150 g/kg锦秋（Phaseolus vulgaris品种）淀粉（KS）或150 g/kg tebou（P. vulgaris品种）淀粉（TS）的无胆固醇饮食，持续4周。整个实验期间，各组大鼠体重无显著差异。CS组大鼠肝脏重量比AS、KS和TS组高1.1 - 1.2倍。TS组大鼠盲肠重量比CS组高1.4倍，且CS组盲肠pH值显著高于其他组。在喂食期间，豆类淀粉组大鼠的血清总胆固醇、极低密度脂蛋白 + 中间密度脂蛋白 + LDL胆固醇和高密度脂蛋白（HDL）胆固醇浓度均显著低于CS组。在4周喂食期结束时，豆类淀粉组的总胆固醇/HDL胆固醇比值也显著低于CS组。在4周喂食期结束时，TS组大鼠肝脏胆固醇浓度显著高于CS组。AS组肝脏载脂蛋白B mRNA的相对量比CS组高1.2倍，AS组和TS组肝脏LDL受体mRNA水平比CS组高1.8 - 2.0倍。本研究结果表明，AS、KS和TS通过提高肝脏LDL受体mRNA水平降低了血清总胆固醇水平。
