Inoue T, Yanagihara S, Misono Y, Suzuki M
Department of Chemistry, Faculty of Science, Fukuoka University, Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Chem Phys Lipids. 2001 Feb;109(2):117-33. doi: 10.1016/s0009-3084(00)00170-5.
The effect of some fatty acids on the phase behavior of hydrated dipalmitoylphosphatidylcholine (DPPC) bilayer was investigated with special interest in possible difference between saturated and unsaturated fatty acids. The phase behavior of hydrated DPPC bilayer was followed by a differential scanning calorimetry and a Fourier transform infrared spectroscopy. The addition of palmitic acid (PA) increased the bilayer phase transition temperature with the increase of the PA content in the mixture. In addition, DPPC molecules in gel phase bilayer became more rigid in the presence of PA compared with those in the absence of PA. This effect of PA on the phase behavior of hydrated DPPC bilayer is common to other saturated fatty acids, stearic acid, myristic acid, and also to unsaturated fatty acid with trans double bond, elaidic acid. Contrary to these fatty acids, oleic acid (OA), the unsaturated fatty acid with cis double bond in the acyl chain, exhibited quite different behavior. The effect of OA on the bilayer phase transition temperature was rather small, although a slight decrease in the temperature was appreciable. Furthermore, the IR spectral results demonstrated that the perturbing effect of OA on the gel phase bilayer of DPPC was quite small. These results mean that OA does not disturb the hydrated DPPC bilayer significantly.
研究了一些脂肪酸对水合二棕榈酰磷脂酰胆碱（DPPC）双层膜相行为的影响，特别关注饱和脂肪酸和不饱和脂肪酸之间可能存在的差异。采用差示扫描量热法和傅里叶变换红外光谱法跟踪水合DPPC双层膜的相行为。随着混合物中棕榈酸（PA）含量的增加，其添加使双层膜的相变温度升高。此外，与不存在PA时相比，在PA存在下凝胶相双层膜中的DPPC分子变得更加刚性。PA对水合DPPC双层膜相行为的这种影响对于其他饱和脂肪酸，如硬脂酸、肉豆蔻酸，以及具有反式双键的不饱和脂肪酸反油酸也是相同的。与这些脂肪酸相反，在酰基链中具有顺式双键的不饱和脂肪酸油酸（OA）表现出截然不同的行为。尽管温度有轻微下降，但OA对双层膜相变温度的影响相当小。此外，红外光谱结果表明，OA对DPPC凝胶相双层膜的扰动作用相当小。这些结果意味着OA不会显著干扰水合DPPC双层膜。
