Yoshinoya S, Mizoguchi Y, Aotsuka S, Yokohari R, Nishioka K, Miyamoto T
Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Japan.
J Clin Lab Immunol. 1992;38(4):161-73.
The authors studied sera from both patients with SLE and from those with RA to evaluate clinical usefulness and significance of circulating immune complexes (CIC) detected with new ELISA kits utilizing monoclonal anti-C1q and anti-C3d antibodies. CIC values of patients with SLE significantly correlated to severity of disease activities evaluated by clinical symptoms and laboratory tests, especially for serum complement levels. Since substances detected with the ELISA kits were closely related to serum complement components, it was determined that a direct relationship exists between clinical activities and CIC values appearing in SLE patients with hypocomplementemia. In RA patients, CIC values did not correlate to clinical activities evaluated by Lansbury's index, anatomical bone damage with X-ray or functional assessment of activities of daily living, but did significantly correlate to levels of IgM-RF, serum IgG concentrations and some markers of systemic inflammation. Detection of IC after fractionation of RA sera revealed a broad range of molecular sizes detectable with the ELISA kits, which indicated that CIC in vivo were heterogenic and complicated in formation, degradation and interaction with serum complements.
作者研究了系统性红斑狼疮（SLE）患者和类风湿关节炎（RA）患者的血清，以评估使用新型酶联免疫吸附测定（ELISA）试剂盒（利用单克隆抗C1q和抗C3d抗体）检测循环免疫复合物（CIC）的临床实用性和意义。SLE患者的CIC值与通过临床症状和实验室检查评估的疾病活动严重程度显著相关，尤其是血清补体水平。由于ELISA试剂盒检测到的物质与血清补体成分密切相关，因此确定在低补体血症的SLE患者中，临床活动与出现的CIC值之间存在直接关系。在RA患者中，CIC值与通过兰斯伯里指数评估的临床活动、X线检查的解剖学骨损伤或日常生活活动功能评估均无相关性，但与IgM类风湿因子（IgM-RF）水平、血清IgG浓度及一些全身炎症标志物显著相关。对RA血清进行分级分离后检测免疫复合物（IC），结果显示ELISA试剂盒可检测到广泛的分子大小，这表明体内CIC在形成、降解及与血清补体相互作用方面具有异质性且很复杂。
