Hermosa M R, Grondona I, Díaz-Mínguez J M, Iturriaga E A, Monte E
Departamento de Microbiología y Genética, Universidad de Salamanca, Spain.
Curr Genet. 2001 Jan;38(6):343-50. doi: 10.1007/s002940000173.
The genus Trichoderma includes biocontrol agents (BCAs) effective against soilborne plant pathogenic fungi. Several potentially useful strains for biological control are difficult to distinguish from other strains of Trichoderma found in the field. So, there is a need to find ways to monitor these strains when applied to natural pathosystems. We have used random amplified polymorphic DNA (RAPD) markers to estimate genetic variation among sixteen strains of the species T. asperellum, T. atroviride, T. harzianum, T. inhamatum and T. longibrachiatum previously selected as BCAs, and to obtain fingerprinting patterns. Analysis of these polymorphisms revealed four distinct groups, in agreement with previous studies. Some of the RAPD products generated were used to design specific primers. Diagnostic PCR performed using these primers specifically identify the strain T. atroviride 11, showing that DNA markers may be successfully used for identification purposes. This SCAR (sequence-characterised amplified region) marker can clearly distinguish strain 11 from other closely related Trichoderma strains.
木霉属包含对土传植物病原真菌有效的生物防治剂（BCAs）。几种具有潜在生物防治用途的菌株很难与田间发现的其他木霉菌株区分开来。因此，当将这些菌株应用于自然病害系统时，有必要找到监测它们的方法。我们使用随机扩增多态性DNA（RAPD）标记来估计先前被选作生物防治剂的16株棘孢木霉、绿色木霉、哈茨木霉、因哈木霉和长枝木霉的遗传变异，并获得指纹图谱。对这些多态性的分析揭示了四个不同的组，这与先前的研究一致。所产生的一些RAPD产物被用于设计特异性引物。使用这些引物进行的诊断性PCR能够特异性鉴定绿色木霉11菌株，表明DNA标记可成功用于鉴定目的。这种序列特征性扩增区域（SCAR）标记能够清楚地区分11菌株与其他密切相关的木霉菌株。
