人类卵巢周期卵泡期和黄体期自然杀伤淋巴细胞的细胞因子产生
Cytokine production by natural killer lymphocytes in follicular and luteal phase of the ovarian cycle in humans.
作者信息
Bouman A, Moes H, Heineman M J, de Leij L F, Faas M M
机构信息
Department of Obstetrics and Gynaecology, University of Groningen and University Hospital, The Netherlands.
出版信息
Am J Reprod Immunol. 2001 Mar;45(3):130-4. doi: 10.1111/j.8755-8920.2001.450302.x.
PROBLEM
The aim of this study was to test the hypothesis that, during luteal phase of the ovarian cycle, as compared with follicular phase, the cytokine productive capacity of peripheral natural killer (NK)-lymphocytes in humans is shifted towards a "Th2-type"-like response.
METHOD OF STUDY
Intracellular Th1 and Th2 cytokine production by in vitro activated peripheral NK-lymphocytes in a whole blood preparation of the follicular and the luteal phase of the ovarian cycle was measured by flow cytometry.
RESULTS
There was no difference in interferon (IFN)-gamma, interleukin (IL)-2, IL-4, and IL-10 cytokine production in activated NK-lymphocytes when comparing luteal phase with follicular phase of the ovarian cycle. However, there was a significant increase in peripheral NK-lymphocyte number in luteal phase compared with follicular phase.
CONCLUSION
The cytokine productive capacity of peripheral NK-lymphocytes is not shifted towards a "Th2-type"-like response in the luteal phase as compared with the follicular phase of the ovarian cycle in humans.
问题
本研究的目的是检验以下假设：在人类卵巢周期的黄体期，与卵泡期相比，外周自然杀伤（NK）淋巴细胞的细胞因子产生能力会转向“Th2型”样反应。
研究方法
通过流式细胞术测量在卵巢周期卵泡期和黄体期的全血制备物中，体外活化的外周NK淋巴细胞产生的细胞内Th1和Th2细胞因子。
结果
比较卵巢周期的黄体期和卵泡期时，活化的NK淋巴细胞中干扰素（IFN）-γ、白细胞介素（IL）-2、IL-4和IL-10细胞因子的产生没有差异。然而，与卵泡期相比，黄体期外周NK淋巴细胞数量显著增加。
结论
与人类卵巢周期的卵泡期相比，黄体期外周NK淋巴细胞的细胞因子产生能力不会转向“Th2型”样反应。
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