Omu Alexander E, Al-Azemi Majedah K, Makhseed Ma'asoumah, Al-Oattan Fawzia, Ismail Amal A, Al-Tahir Sami, Al-Busiri Nasser
Department of Obstetrics and Gynecology, Faculty of Medicine, Maternity Hospital, Kuwait University, PO Box 24923 Safat, 13110 Kuwait.
Acta Obstet Gynecol Scand. 2003 Jul;82(7):603-9. doi: 10.1034/j.1600-0412.2003.00045.x.
Estrogen and progesterone immunoregulate the genital environment by expression of cytokines and growth factors.
To investigate the pattern of expression of T-helper cytokines during the ovarian cycle compared with women with chronic anovulation resistant to clomiphene citrate.
Expression of T-helper cytokines in women with chronic anovulation may be different from the pattern in women with a normal ovarian cycle.
We evaluated 31 infertile women having laparoscopy for evaluation of tubal patency and evidence of ovulation in two groups during (a) the luteal phase (17 women) and (b) the follicular phase (14 women). A third group was composed of 14 women with polycystic ovarian syndrome, but they were resistant to clomiphene citrate for induction of ovulation and had laparoscopic ovarian cautery. Peritoneal fluid was collected during laparoscopy. Estimation of T-helper cytokine interleukin (IL)-2, tumor necrosis factor (TNF)-alpha, IL-4 and IL-6 in serum, peritoneal fluid and culture of the peritoneal mononuclear cells was performed by ELISA. Serum luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin, estradiol and progesterone were evaluated by the Vidas Parametric System.
The LH : FSH ratio was significantly higher in the women with polycystic ovaries than in the ovulatory groups. IL-2 and IFN-gamma were more highly expressed in the follicular phase but the T-helper 2 cytokines IL-4 and IL-6 predominated in the luteal phase, serum, peritoneal fluid and culture of the peritoneal mononuclear cells. From the follicular to the mid-luteal phase, IL-6 increased three to fivefold in the serum and peritoneal fluid, but there was low expression with anovulation.
The peritoneal fluid levels of IL-4 and IL-6 are higher in the luteal phase. Low IL-6 levels in chronic anovulation may be a marker of resistance to clomiphene citrate.
雌激素和孕激素通过细胞因子和生长因子的表达对生殖系统环境进行免疫调节。
与对枸橼酸氯米芬耐药的慢性无排卵女性相比，研究卵巢周期中辅助性T细胞细胞因子的表达模式。
慢性无排卵女性中辅助性T细胞细胞因子的表达模式可能与正常卵巢周期女性不同。
我们评估了31例因不孕症接受腹腔镜检查以评估输卵管通畅性及排卵情况的女性，分为两组：(a)黄体期（17例女性）和(b)卵泡期（14例女性）。第三组由14例多囊卵巢综合征女性组成，她们对枸橼酸氯米芬诱导排卵耐药且接受了腹腔镜下卵巢烧灼术。在腹腔镜检查期间收集腹腔液。通过酶联免疫吸附测定法对血清、腹腔液及腹腔单核细胞培养物中的辅助性T细胞细胞因子白细胞介素（IL）-2、肿瘤坏死因子（TNF）-α、IL-4和IL-6进行测定。通过Vidas参数系统评估血清促黄体生成素（LH）、促卵泡生成素（FSH）、催乳素、雌二醇和孕酮水平。
多囊卵巢女性的LH:FSH比值显著高于排卵组。IL-2和干扰素-γ在卵泡期表达更高，但辅助性T细胞2型细胞因子IL-4和IL-6在黄体期的血清、腹腔液及腹腔单核细胞培养物中占主导。从卵泡期到黄体中期，血清和腹腔液中的IL-6增加了三到五倍，但无排卵时表达较低。
黄体期腹腔液中IL-4和IL-6水平较高。慢性无排卵时IL-6水平较低可能是对枸橼酸氯米芬耐药的一个标志。
