Dervilly-Pinel G, Thibault J F, Saulnier L
Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, INRA, B.P. 71627, Nantes, France.
Carbohydr Res. 2001 Feb 15;330(3):365-72. doi: 10.1016/s0008-6215(00)00300-1.
Purified water-soluble arabinoxylans from wheat flour were deferuloylated and fractionated into six fractions by graded ethanol precipitation. Further fractionation by HPSEC on Sephacryl S500 resulted in 48 subfractions with low polydispersity index. Conformational characteristics (persistence length q, hydrodynamic parameter v and Mark-Houwink exponent a) were similar among all subfractions and fitted with a semi-flexible conformation, whatever their structural characteristics. Substitution degree of the xylan backbone by arabinose residues has no influence on the conformation of arabinoxylans.
从小麦粉中纯化得到的水溶性阿拉伯木聚糖进行脱阿魏酸处理，并通过分级乙醇沉淀法分为六个级分。在Sephacryl S500上进行高效尺寸排阻色谱（HPSEC）进一步分级，得到48个具有低多分散指数的亚级分。无论其结构特征如何，所有亚级分的构象特征（持久长度q、流体动力学参数v和Mark-Houwink指数a）相似，符合半柔性构象。阿拉伯糖残基对木聚糖主链的取代度对阿拉伯木聚糖的构象没有影响。
