De Anda-Flores Yubia, Carvajal-Millan Elizabeth, Lizardi-Mendoza Jaime, Rascon-Chu Agustin, Tanori-Cordova Judith, Martínez-López Ana Luisa, Burgara-Estrella Alexel J, Pedroza-Montero Martin R
Biopolymers-CTAOA, Research Center for Food and Development (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán Rosas No. 46, Hermosillo 83304, Mexico.
Biotechnology-CTAOV, Research Center for Food and Development (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán Rosas No. 46, Hermosillo 83304, Mexico.
Polymers (Basel). 2021 Aug 20;13(16):2794. doi: 10.3390/polym13162794.
This study aimed to investigate the effect of arabinoxylans (AX) partial de-esterification with feruloyl esterase on the polysaccharide conformational behavior, topographical features, and antioxidant activity. After enzyme treatment, the ferulic acid (FA) content in AX was reduced from 7.30 to 5.48 µg FA/mg polysaccharide, and the molecule registered a small reduction in radius of gyration (RG), hydrodynamic radius (Rh), characteristic ratio (C∞), and persistence length (q). A slight decrease in α and a small increase in K constants in the Mark-Houwink-Sakurada equation for partially de-esterified AX (FAX) suggested a reduction in molecule structural rigidity and a more expanded coil conformation, respectively, in relation to AX. Fourier transform infrared spectroscopy spectra of AX and FAX presented a pattern characteristic for this polysaccharide. Atomic force microscopy topographic analysis of FAX showed a more regular surface without larger hollows in relation to AX. The antioxidant activity of FAX, compared to AX, was reduced by 30 and 41% using both 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) methods, respectively. These results suggest that feruloyl esterase treatment of AX could offer a strategy to tailor AX chains conformation, morphological features, and antioxidant activity, impacting the development of advanced biomaterials for biomedical and pharmaceutical applications.
本研究旨在探讨阿魏酸酯酶对阿拉伯木聚糖（AX）进行部分脱酯作用后，对多糖构象行为、形貌特征及抗氧化活性的影响。酶处理后，AX中的阿魏酸（FA）含量从7.30 μg FA/mg多糖降至5.48 μg FA/mg多糖，分子的回转半径（RG）、流体力学半径（Rh）、特征比（C∞）和持久长度（q）略有减小。部分脱酯阿拉伯木聚糖（FAX）的Mark-Houwink-Sakurada方程中α值略有下降，K常数略有增加，这分别表明相对于AX，分子结构刚性降低，线圈构象更加伸展。AX和FAX的傅里叶变换红外光谱呈现出该多糖的特征图谱。与AX相比，FAX的原子力显微镜形貌分析显示其表面更规则，没有较大的空洞。分别使用2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和1,1-二苯基-2-苦基肼（DPPH）方法测定，FAX的抗氧化活性相对于AX分别降低了30%和41%。这些结果表明，用阿魏酸酯酶处理AX可为调整AX链的构象、形态特征和抗氧化活性提供一种策略，从而影响用于生物医学和制药应用的先进生物材料的开发。
