汉防己甲素对豚鼠离体回肠吗啡依赖性的影响。
Effect of tetrandrine on morphine dependence in isolated guinea pig ileum.
作者信息
Liu Z H, Li J L, Li N, Tian C L, Zhang K G
机构信息
Department of Pharmacology, Binzhou Medical College, Binzhou 256603, China.
出版信息
Zhongguo Yao Li Xue Bao. 1999 Nov;20(11):1000-4.
AIM
To evaluate the effects of tetrandrine (Tet) and nimodipine (Nim) on the morphine (Mor) withdrawal response in the isolated guinea pig ileum.
METHODS
The withdrawal contracture was elicited by addition of naloxone (Nal) (1 mumol.L-1) to the isolated naive ileum incubated with Mor (3 mumol.L-1) at 37.5 degrees C for 4 h or to the ileum obtained from Mor-dependent guinea pig.
RESULTS
When Nim (0.01, 0.05, and 0.1 mumol.L-1) or Tet (1, 10, and 50 mumol.L-1) was added 1 min before Nal in the naive ilea bathed in Krebs solution containing Mor, or when the ilea from Mor-dependent guinea pigs were incubated with Nim (0.01, 0.05, and 0.1 mumol.L-1) or Tet (1, 10, and 50 mumol.L-1) for 15 min, or when Nim (5 and 10 mg.kg-1, i.p.) or Tet (15 and 30 mg.kg-1, i.p.) was administered in vivo to Mor-dependent guinea pigs, the Nal-precipitated withdrawal contracture was significantly decreased in a dose-dependent manner.
CONCLUSION
Tet and Nim, Ca2+ channel blockers, could inhibit the Nal-precipitated Mor withdrawal response in the isolated guinea pig ileum.
目的
评估粉防己碱（Tet）和尼莫地平（Nim）对离体豚鼠回肠吗啡（Mor）戒断反应的影响。
方法
将离体的正常回肠在37.5℃下与Mor（3 μmol·L-1）孵育4小时，或从吗啡依赖的豚鼠获取回肠，加入纳洛酮（Nal）（1 μmol·L-1）引发戒断挛缩。
结果
在含有Mor的Krebs溶液中孵育的正常回肠中，在加入Nal前1分钟加入Nim（0.01、0.05和0.1 μmol·L-1）或Tet（1、10和50 μmol·L-1），或者将吗啡依赖豚鼠的回肠与Nim（0.01、0.05和0.1 μmol·L-1）或Tet（1、10和50 μmol·L-1）孵育15分钟，或者对吗啡依赖的豚鼠进行Nim（5和10 mg·kg-1，腹腔注射）或Tet（15和30 mg·kg-1，腹腔注射）的体内给药时，Nal诱发的戒断挛缩均以剂量依赖方式显著降低。
结论
Ca2+通道阻滞剂Tet和Nim可抑制离体豚鼠回肠中Nal诱发的Mor戒断反应。
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