Wang Cai-yun, Mo Zhi-xian, Tu Hong-hai
Teaching and Research Section of Pharmacology and Medicinal Chemistry, Department of Traditional Chinese Medicine, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2003 Apr;23(4):329-31.
To investigate the effects of sinomenine on morphine withdrawal response and acetylchline (Ach)-induced contracture in isolated guinea pig ileum.
The withdrawal contracture was elicited by subjecting isolated ileum incubated with morphine (3 micromol/L) at 37.5 degrees Celsius for 4 h to naloxone (1 micromol/L) treatment. Sinomenine (10, 50, 250 micromol/L) and nimodipine (Nim, 0.1 micromol/L) were administered 1 min before and after naloxone in morphine-dependent ilea bathed in Krebs solution containing morphine, to observe the changes in the withdrawal contracture of the ileum. The effect of sinomenine (10, 50, 250 micromol/L) on the contracture of untreated ileum in Krebs solution elicited by acetylcholine was also observed.
Naloxone-induced withdrawal contracture or acetylcholine-induced contracture of the ileum was significantly decreased in a dose-dependent manner, indicating that sinomenine can inhibit morphine withdrawal symptoms in guinea pigs.
研究青藤碱对吗啡戒断反应及豚鼠离体回肠乙酰胆碱（Ach）诱导的挛缩的影响。
将离体回肠在37.5摄氏度下与3微摩尔/升吗啡孵育4小时，然后用1微摩尔/升纳洛酮处理以引发戒断挛缩。在含有吗啡的Krebs溶液中，于吗啡依赖的回肠给予纳洛酮前后1分钟，分别给予青藤碱（10、50、250微摩尔/升）和尼莫地平（Nim，0.1微摩尔/升），观察回肠戒断挛缩的变化。还观察了青藤碱（10、50、250微摩尔/升）对Krebs溶液中未处理回肠乙酰胆碱诱导挛缩的影响。
纳洛酮诱导的回肠戒断挛缩或乙酰胆碱诱导的挛缩均呈剂量依赖性显著降低，表明青藤碱可抑制豚鼠的吗啡戒断症状。
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