Zhang X Q, Wang B, Zhang M Y, Xiao J G
Department of Pharmacology, Nanjing Medical University, Nanjing 210029, China.
Zhongguo Yao Li Xue Bao. 1999 Oct;20(10):893-6.
To study the effects of TMB-8 on [Ca2+]i elevation induced by neurotransmitters in dissociated brain cells.
The brain cell suspension was made using a gentle trituration for 1 min with a polished pipette. The changes of [Ca2+]i were detected by the fluorescent indicator, Fura 2-AM.
In the presence of extracellular Ca2+ 1.3 mmol.L-1, sodium glutamate (Glu), histamine (His), and serotonin (5-HT) markedly increased the [Ca2+]i which were reduced by TMB-8 30 mumol.L-1. TMB-8 3 mumol.L-1 produced inhibitory effects on the increase of [Ca2+]i by His and 5-HT in a Ca(2+)-free Hanks' solution. The increase of [Ca2+]i by His and 5-HT was reduced to control level by TMB-8 10 mumol.L-1.
TMB-8 inhibited the [Ca2+]i elevation induced by Glu, 5-HT, and His in brain cells.
研究三甲基溴化硼（TMB - 8）对离体脑细胞中神经递质诱导的细胞内钙离子浓度（[Ca2+]i）升高的影响。
用抛光移液管轻柔研磨1分钟制备脑细胞悬液。采用荧光指示剂Fura 2 - AM检测[Ca2+]i的变化。
在细胞外钙离子浓度为1.3 mmol·L-1时，谷氨酸（Glu）、组胺（His）和5 - 羟色胺（5 - HT）显著升高[Ca2+]i，而30 μmol·L-1的TMB - 8可使其降低。在无钙的汉克斯溶液中，3 μmol·L-1的TMB - 8对His和5 - HT引起的[Ca2+]i升高产生抑制作用。10 μmol·L-1的TMB - 8可使His和5 - HT引起的[Ca2+]i升高降至对照水平。
TMB - 8抑制脑细胞中Glu、5 - HT和His诱导的[Ca2+]i升高。
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