8-（N，N-二乙氨基）-正辛基-3,4,5-三甲氧基苯甲酸酯可降低组胺、5-羟色胺和谷氨酸诱导的培养小牛基底动脉平滑肌细胞内[Ca2+]i升高。
8-(N,N-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate reduced [Ca2+]i elevation induced by histamine, serotonin, and glutamate in cultured calf basilar artery smooth muscle cells.
作者信息
Wang B, Zhang X Q, Liu T P, Xiao J G
机构信息
Department of Pharmacology, Nanjing Medical University, China.
出版信息
Zhongguo Yao Li Xue Bao. 1998 May;19(3):251-3.
AIM
To study the effects of 8-(N,N-diethylamino)-n-octyl-3,4,5- trimethoxybenzoate (TMB-8) on intracellular free calcium ([Ca2+]i) in cultured calf basilar artery smooth muscle cells.
METHODS
[Ca2+]i was examined by a system of measurement of AR-CM-MIC, using Fura 2-AM as a fluorescent indicator.
RESULTS
In the presence of extracellular Ca2+ 1.3 mmol.L-1, histamine (His), serotonin (5-HT), and sodium glutamate (Glu) markedly increased the [Ca2+]i which was attenuated by TMB-8. In Ca2+ free Hanks' solution containing egtazic acid 0.1 mmol.L-1, TMB-8 not only reduced the resting [Ca2+]i, but also inhibited the elevation of [Ca2+]i evoked by His and 5-HT.
CONCLUSION
TMB-8 reduced the resting [Ca2+]i and attenuated His-, 5-HT-, and Glu-induced increases of [Ca2+]i in basilar artery smooth muscle cells.
目的
研究8 - （N，N - 二乙氨基） - 正辛基 - 3,4,5 - 三甲氧基苯甲酸酯（TMB - 8）对培养的小牛基底动脉平滑肌细胞内游离钙（[Ca2+]i）的影响。
方法
以Fura 2 - AM作为荧光指示剂，采用AR - CM - MIC测量系统检测[Ca2+]i。
结果
在细胞外Ca2+浓度为1.3 mmol.L-1时，组胺（His）、5 - 羟色胺（5 - HT）和谷氨酸钠（Glu）可显著升高[Ca2+]i，而TMB - 8可使其降低。在含有0.1 mmol.L-1依他酸的无Ca2+ Hank's溶液中，TMB - 8不仅可降低静息[Ca2+]i，还可抑制His和5 - HT引起的[Ca2+]i升高。
结论
TMB - 8可降低基底动脉平滑肌细胞的静息[Ca2+]i，并减弱His、5 - HT和Glu诱导的[Ca2+]i升高。
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