Ohdan H, Suzuki S, Kanashiro M, Amemiya H, Fukuda Y, Dohi K
Second Department of Surgery, Hiroshima University, School of Medicine, Japan.
Transpl Int. 1994;7 Suppl 1:S542-6. doi: 10.1111/j.1432-2277.1994.tb01439.x.
We established a new technique of in vivo near-infrared (NIR) spectroscopy that can estimate both nitric oxide (NO) production and tissue oxygen sufficiency in living organs during the alloimmune response. The present study was aimed at evaluating the potential of this technique for monitoring the rejection response utilizing the rat model of orthotopic liver transplantation without arterialization. The relative changes of nitrosyl-hemoglobin, oxyhemoglobin and oxidized-cytochrome oxidase in the graft livers were quantified by use of this method. Nitrosyl-hemoglobin in the allogenic grafts was elevated at the onset of the rejection response and was suppressed when the rejection reaction was treated by the administration of 15-deoxyspergualin. Oxy-hemoglobin and oxidized-cytochrome oxidase were decreased in accordance with parenchymal disorder determined histologically. These results demonstrated that the new technique of in vivo NIR spectroscopy can assess simultaneously both the immune response and graft function after liver transplantation.
我们建立了一种新的体内近红外（NIR）光谱技术，该技术能够在同种免疫反应期间评估活体器官中一氧化氮（NO）的生成以及组织氧充足情况。本研究旨在利用非动脉化原位肝移植大鼠模型评估该技术监测排斥反应的潜力。通过使用该方法对移植肝脏中亚硝基血红蛋白、氧合血红蛋白和氧化型细胞色素氧化酶的相对变化进行了定量分析。同种异体移植物中的亚硝基血红蛋白在排斥反应开始时升高，而在给予15-去氧精胍菌素治疗排斥反应时受到抑制。氧合血红蛋白和氧化型细胞色素氧化酶根据组织学确定的实质紊乱而降低。这些结果表明，新的体内NIR光谱技术能够同时评估肝移植后的免疫反应和移植物功能。
