Ohdan H, Fukuda Y, Suzuki S, Amemiya H, Dohi K
Second Department of Surgery, Hiroshima University School of Medicine, Japan.
Transplantation. 1995 Sep 27;60(6):530-5. doi: 10.1097/00007890-199509270-00002.
Near-infrared (NIR) spectroscopy was applied to rat liver allografts for assessing nitric oxide (NO) synthesis and tissue oxygenation as a means of monitoring the rejection response following liver transplantation. Orthotopic liver transplantation was performed in rats, which were assigned to three groups as follows: group 1, a syngeneic combination (lewis to Lewis); group 2, an allogeneic combination (ACI to Lewis); and group 3, an allogeneic combination treated with 15-deoxyspergualin. NIR spectroscopy was performed on the grafts in recipients, and the relative changes in nitrosyl-Hb (NO bound to erythrocyte hemoglobin), oxy-Hb, and oxidized cytochrome oxidase (Cyt.aa3) were obtained. The level of nitrosyl-Hb was significantly elevated from postoperative day (POD) 3 in group 2 compared with that in group 1, which remained constant (P < 0.05). In group 3, the elevation was significantly suppressed. These data indicate that the alloimmune response is associated with a dramatic change in NO synthesis in grafted livers. In a separate experiment, NO synthesis was also increased after long cold preservation (24 hr) in syngeneic liver transplants. However, the increase was transient and subsided on POD 3. Levels of oxy-Hb and oxidized Cyt.aa3 in group 2 were significantly decreased when parenchymal disorder was confirmed histologically (POD 6 and 8), compared with those in group 1, which remained constant (P < 0.05). In group 3, both of these levels showed improvement. Thus, our NIR spectroscopy technique was shown to be capable of assessing simultaneously both the immune response and the degree of immune-induced destruction of allograft tissue following liver transplantation through monitoring of NO synthesis and tissue oxygenation.
近红外（NIR）光谱技术被应用于大鼠肝脏同种异体移植，以评估一氧化氮（NO）合成和组织氧合情况，作为监测肝移植后排斥反应的一种手段。对大鼠进行原位肝移植，并将其分为以下三组：第1组为同基因组合（Lewis大鼠之间）；第2组为异基因组合（ACI大鼠到Lewis大鼠）；第3组为用15-去氧精胍菌素处理的异基因组合。对受体的移植肝脏进行近红外光谱检测，获得亚硝基血红蛋白（与红细胞血红蛋白结合的NO）、氧合血红蛋白和氧化细胞色素氧化酶（Cyt.aa3）的相对变化。与第1组（保持稳定）相比，第2组术后第3天亚硝基血红蛋白水平显著升高（P<0.05）。在第3组中，这种升高被显著抑制。这些数据表明，同种免疫反应与移植肝脏中NO合成的显著变化有关。在另一项实验中，同基因肝移植经长时间冷保存（24小时）后NO合成也增加。然而，这种增加是短暂的，在术后第3天就消退了。当组织学证实实质紊乱时（术后第6天和第8天），与第1组（保持稳定）相比，第2组的氧合血红蛋白和氧化Cyt.aa3水平显著降低（P<0.05）。在第3组中，这两个水平均有所改善。因此，我们的近红外光谱技术通过监测NO合成和组织氧合情况，能够同时评估肝移植后同种异体移植组织的免疫反应和免疫诱导破坏程度。
