Rosenkrands I, King A, Weldingh K, Moniatte M, Moertz E, Andersen P
Department of TB Immunology, Statens Serum Institute, Copenhagen, Denmark.
Electrophoresis. 2000 Nov;21(17):3740-56. doi: 10.1002/1522-2683(200011)21:17<3740::AID-ELPS3740>3.0.CO;2-3.
Human tuberculosis is caused by the intracellular pathogen Mycobacterium tuberculosis. Sequencing of the genome of M. tuberculosis strain H37Rv has predicted 3924 open reading frames, and enabled identification of proteins from this bacterium by peptide mass fingerprinting. Extracellular proteins from the culture medium and proteins in cellular extracts were examined by two-dimensional gel electrophoresis using immobilized pH gradient technology. By mass spectrometry and immunodetection, 49 culture filtrate proteins and 118 lysate proteins were identified, 83 of which were novel. To date, 288 proteins have been identified in M. tuberculosis proteome studies, and a list is presented which includes all identified proteins (available at http://www.ssi.dk/publichealth/tbimmun). The information obtained from the M. tuberculosis proteome so far is discussed in relation to the information obtained from the complete genome sequence.
人类结核病由细胞内病原体结核分枝杆菌引起。结核分枝杆菌H37Rv菌株的基因组测序预测有3924个开放阅读框，并通过肽质量指纹图谱技术实现了对该细菌蛋白质的鉴定。使用固定化pH梯度技术，通过二维凝胶电泳对培养基中的细胞外蛋白质和细胞提取物中的蛋白质进行了检测。通过质谱分析和免疫检测，鉴定出49种培养滤液蛋白和118种裂解物蛋白，其中83种是新发现的。迄今为止，在结核分枝杆菌蛋白质组研究中已鉴定出288种蛋白质，并列出了一份包含所有已鉴定蛋白质的清单（可在http://www.ssi.dk/publichealth/tbimmun获取）。本文结合从完整基因组序列中获得的信息，对目前从结核分枝杆菌蛋白质组中获得的信息进行了讨论。
